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ligand
lentil lectin
包装
pkg of 25 mL
制造商/商品名称
Cytiva 17-0444-01
储存条件
(20% Ehtanol, PBS pH 7)
基质
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
3-12
工作范围
3-10
储存温度
2-8°C
一般描述
Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose™ 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.
特点和优势
- Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
- Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
- Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
- Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
储存及稳定性
分析说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。
This page shows how to prepare samples for purification with affinity chromatography.
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