ligand
concanavalin A (con A)
包装
pack of 100 mL
制造商/商品名称
Cytiva 17-0440-01
储存条件
(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
基质
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
4-9
working range
4-6
储存温度
2-8°C
相关类别
一般描述
Con A Sepharose™ is Concanavalin A coupled to Sepharose™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.
特点和优势
- Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Group-specific adsorbent for molecules containing sugars.
- Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
- Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
- Con A coupled to Sepharose™ 4B via cyanogen bromide activation
储存及稳定性
分析说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。
This page shows how to prepare samples for purification with affinity chromatography.
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