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安全信息

GE17-0440-01

Con-A Sepharose 4B

Cytiva 17-0440-01, pack of 100 mL

别名:

Concanavalin A Sepharose

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

ligand

concanavalin A (con A)

包装

pack of 100 mL

制造商/商品名称

Cytiva 17-0440-01

储存条件

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

4-9

working range

4-6

储存温度

2-8°C

相关类别

一般描述

Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.

特点和优势

  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars.
  • Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
  • Con A coupled to Sepharose 4B via cyanogen bromide activation

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

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This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。

This page shows how to prepare samples for purification with affinity chromatography.

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