所有图片(1)

主要文件

COO原产地证书/COA分析证书

安全信息

安全和法规

GE17-0409-03

HiTrap^® Chelating High Performance

Name: HiTrap<SUP>®</SUP> Chelating High Performance
Brand: SIGMA

Cytiva 17-0409-03, pack of 5 × 5 mL

别名:

HiTrap Chelating Column

登录查看公司和协议定价

所有图片(1)

About This Item

UNSPSC代码:

23151816

NACRES:

NA.56

推荐产品

Slide 1 of 10

1 of 10

GE17-0408-01

HiTrap^® Chelating High Performance

GE17-5255-01

HisTrap^™ Fast Flow

Sigma-Aldrich

GE28-9893-35

HiLoad^® 16/600 Superdex^® 200 pg

GE17-0409-01

HiTrap^® Chelating High Performance

Sigma-Aldrich

GE17-5087-01

HiPrep^™ 26/10 Desalting

Sigma-Aldrich

GE18-1153-17

TRICORN 10/200 GLASS COLUMN

Millipore

UFC8030

Amicon^® Ultra离心超滤管，30 kDa MWCO

GE17-1154-01

HiTrap^® Q High Performance

GE17-5248-01

HisTrap^™高性能

GE17-5247-01

HisTrap^™ High Performance

ligand

iminodiacetic acid

包装

pack of 5 × 5 mL

制造商/商品名称

Cytiva 17-0409-03

参数

<20 mL/min flow rate
42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

尺寸

平均零件尺寸

34 μm

cleaning

2-14

工作范围

3-13

一般描述

HiTrap^® Chelating HP is prepacked with Chelating Sepharose^™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

HiTrap^® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC^™ Systems.

特点和优势

For optimizing purification of histidine-tagged proteins using different kinds of metal ions.
Conveniently charge with your metal of choice.
Iminidoacetic acid coupled to Sepharose^™ High Performance.
Purifying proteins with exposed histidine groups via metal ion complex formation.
Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC^™ System.
Packed with Chelating Sepharose^™ High Performance
Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC^™ System

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.