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Merck
CN

G7396

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

类型

Type IX-A

质量水平

形式

lyophilized powder

比活

1,000,000-5,000,000 units/g protein (30 min assay)

分子量

290 kDa

组成

Protein, 30-70% biuret

运输

wet ice

储存温度

−20°C

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相关类别

应用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据,关于使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。

质量

含有缓冲盐和稳定剂。

单位定义

一个Sigma或改良Fishman单位是指在pH 6.8、温度37°C条件下(30分钟测定),每小时从苯酚葡萄糖醛酸中释放出1.0 μg酚酞所需的酶量。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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商品

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

实验方案

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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