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安全信息

G7396

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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About This Item

CAS号:
9001-45-0
EC 号:
3.2.1.31 (BRENDA, IUBMB)
EC 号:
232-606-8
MDL编号:
MFCD00130629
UNSPSC代码:
12352204
NACRES:
NA.54

类型

Type IX-A

质量水平

300

表单

lyophilized powder

比活

1,000,000-5,000,000 units/g protein (30 min assay)

分子量

290 kDa

组成

Protein, 30-70% biuret

运输

wet ice

储存温度

−20°C

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相关类别

应用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。
用于尿液代谢物分析中葡糖苷酸结合物的水解

质量

含有缓冲盐和稳定剂。

单位定义

一个Sigma或改良Fishman单位是指在pH 6.8、温度37°C条件下（30分钟测定），每小时从苯酚葡萄糖醛酸中释放出1.0 μg酚酞所需的酶量。

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说明
价格

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产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

预防措施声明

P261 - P280 - P342 + P311

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000398556
0000398552
0000398557
0000398555
0000398558

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Bojan Šarkanj et al.
Analytica chimica acta, 1019, 84-92 (2018-04-08)
There is a critical need to better understand the patterns, levels and combinatory effects of exposures we are facing through our diet and environment. Mycotoxin mixtures are of particular concern due to chronic low dose exposures caused by naturally contaminated
Maria Papageorgiou et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 35(10), 2032-2044 (2018-08-18)
Deoxynivalenol (DON) is a Fusarium toxin, to which humans are frequently exposed via diet. Although the elderly are speculated to be sensitive to the toxic effects of DON as a result of age-related conditions, disease and altered DON metabolism, there
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine
Health Laboratory Science, 50, 286-289 (2004)
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Desirée Bartolini et al.
Antioxidants (Basel, Switzerland), 10(2) (2021-01-29)
The metabolism of α-tocopherol (α-TOH, vitamin E) shows marked interindividual variability, which may influence the response to nutritional and therapeutic interventions with this vitamin. Recently, new metabolomics protocols have fostered the possibility to explore such variability for the different metabolites
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Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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