G7017
β-葡萄糖醛酸酶 来源于罗曼蜗牛
Type HP-2, aqueous solution, ≥100,000 units/mL
别名:
β-葡萄糖醛酸苷酶
产品名称
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type HP-2, aqueous solution, ≥100,000 units/mL
biological source
Helix pomatia
type
Type HP-2
form
aqueous solution
specific activity
≥100,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Analysis Note
来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
Application
- 通过消化与葡萄糖醛酸苷结合的化合物而制备的LC / MS样品，用于生物标志物和毒理学研究。
- 去除葡萄糖醛酸苷结合物，例如用于制备黄芩素。
Biochem/physiol Actions
内源性β-葡糖醛酸糖苷酶是一种溶酶体酶，参与了葡萄糖醛酸苷修饰的分解代谢产物的消化。
在微生物群中发现的微生物β-葡糖醛酸糖苷酶可以通过水解异种生物质和内生代谢物的葡萄糖醛酸苷结合物而引起代谢物的重吸收。
在微生物群中发现的微生物β-葡糖醛酸糖苷酶可以通过水解异种生物质和内生代谢物的葡萄糖醛酸苷结合物而引起代谢物的重吸收。
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
Features and Benefits
- 最适pH 4.0 - 5.0
- 硫酸酯酶最适pH 6.2
- 硫酸酯酶活性低（<7,500单位/ mL）。
- 硫酸铵悬浮液。
- 来自软体动物的β-葡糖醛酸糖苷酶。
- 可用于低pH样品中葡萄糖醛酸苷的酸水解。
General description
β-来自玛瑙螺的H-2型葡糖醛酸糖苷酶是从罗马蜗牛的消化液中提取的酶的粗溶液。β-来自软体动物的葡糖醛酸糖苷酶也有硫酸酯酶活性。两种活性都可用于代谢物的水解，用于毒理学和生物标志物分析。如需更广泛地了解我们的β-葡糖醛酸糖苷酶产品线及其用途，请参阅我们有关药物代谢物分析的页面
Other Notes
与G0876相似，只是由Sigma生产的。
在pH 5.0、温度37°C条件下，一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞（30分钟实验）。
硫酸酯酶单元定义：一单位的硫酸酯酶每小时，pH5.0，37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
Physical form
在~1.0 M硫酸铵中含有3 mM叠氮化钠作为保存剂的水相溶液。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
法规信息
低风险生物材料
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商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
实验方案
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。
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