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生物来源
rabbit muscle
质量水平
类型
Type I
形式
ammonium sulfate suspension
比活
100-300 units/mg protein
储存条件
(Tightly closed)
技术
activity assay: suitable
颜色
white
异质活性
Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%
储存温度
2-8°C
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相关类别
一般描述
α-磷酸甘油脱氢酶是一种膜蛋白，是黄素连接的初级脱氢酶。
研究领域：细胞信号传导
研究领域：细胞信号传导
应用
α-磷酸甘油脱氢酶（来源于兔肌肉）已用于：
- 配制反应混合物检测甘油激酶活性
- 证明NADH参与的区室化酶反应
- L-墨角藻糖-1-磷酸醛缩酶的反应混合物检测
生化/生理作用
α-磷酸甘油脱氢酶催化L-α-磷酸甘油(Glp)氧化形成磷酸二羟丙酮(DHAP)和过氧化氢(H2O2)。该酶参与呼吸电子传递链、糖酵解和磷脂代谢。
单位定义
在 pH 7.4，25℃ 下，一单位酶每分钟将 1.0 μmole 磷酸二羟丙酮转化为 α-甘油磷酸酯。
外形
溶于 3.2M（NH4）2SO4 和 0.1g/L EDTA 溶液中的结晶悬浮液，pH 6.0
制备说明
根据改良的 Fondy 法进行制备。
分析说明
缩二脲法测定蛋白
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警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Joanne I Yeh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3280-3285 (2008-02-26)
Sn-glycerol-3-phosphate dehydrogenase (GlpD) is an essential membrane enzyme, functioning at the central junction of respiration, glycolysis, and phospholipid biosynthesis. Its critical role is indicated by the multitiered regulatory mechanisms that stringently controls its expression and function. Once expressed, GlpD activity
Structural studies on nicotinamide adenine dinucleotide-linked L-glycerol 3-phosphate dehydrogenase crystallized from rat skeletal muscle.
T P Fondy et al.
The Journal of biological chemistry, 243(11), 3148-3160 (1968-06-10)
Somchart Maenpuen et al.
The FEBS journal, 282(16), 3043-3059 (2015-02-26)
L-α-glycerophosphate oxidase is an FAD-dependent enzyme that catalyzes the oxidation of L-α-glycerophosphate (Glp) by molecular oxygen to generate dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). The catalytic properties of recombinant His6-GlpO from Mycoplasma pneumoniae (His6-MpGlpO) were investigated through transient and
Optimal conditions for biomass and recombinant glycerol kinase production using the yeast Pichia pastoris
Aizemberg R, et al.
Food Technology and Biotechnology, 49(3), 329-329 (2011)
Susan K Boehlein et al.
The Plant journal : for cell and molecular biology, 99(1), 23-40 (2019-02-13)
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration of dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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