G6751
α-甘油磷酸脱氢酶 来源于兔肌肉
Type I, ammonium sulfate suspension, 100-300 units/mg protein
别名:
sn-甘油-3-磷酸:NAD+ 2-氧化还原酶, sn-甘油-3-磷酸脱氢酶 来源于兔肌肉
产品名称
α-甘油磷酸脱氢酶 来源于兔肌肉, Type I, ammonium sulfate suspension, 100-300 units/mg protein
biological source
rabbit muscle
type
Type I
form
ammonium sulfate suspension
specific activity
100-300 units/mg protein
storage condition
(Tightly closed)
technique(s)
activity assay: suitable
color
white
foreign activity
Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%
storage temp.
2-8°C
Quality Level
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相关类别
Analysis Note
缩二脲法测定蛋白
Application
α-磷酸甘油脱氢酶（来源于兔肌肉）已用于：
- 配制反应混合物检测甘油激酶活性
- 证明NADH参与的区室化酶反应
- L-墨角藻糖-1-磷酸醛缩酶的反应混合物检测
Biochem/physiol Actions
α-磷酸甘油脱氢酶催化L-α-磷酸甘油(Glp)氧化形成磷酸二羟丙酮(DHAP)和过氧化氢(H2O2)。该酶参与呼吸电子传递链、糖酵解和磷脂代谢。
General description
α-磷酸甘油脱氢酶是一种膜蛋白，是黄素连接的初级脱氢酶。
研究领域：细胞信号传导
研究领域：细胞信号传导
Other Notes
在 pH 7.4，25℃ 下，一单位酶每分钟将 1.0 μmole 磷酸二羟丙酮转化为 α-甘油磷酸酯。
Physical form
溶于 3.2M（NH4）2SO4 和 0.1g/L EDTA 溶液中的结晶悬浮液，pH 6.0
Preparation Note
根据改良的 Fondy 法进行制备。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Somchart Maenpuen et al.
The FEBS journal, 282(16), 3043-3059 (2015-02-26)
L-α-glycerophosphate oxidase is an FAD-dependent enzyme that catalyzes the oxidation of L-α-glycerophosphate (Glp) by molecular oxygen to generate dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). The catalytic properties of recombinant His6-GlpO from Mycoplasma pneumoniae (His6-MpGlpO) were investigated through transient and
Joanne I Yeh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3280-3285 (2008-02-26)
Sn-glycerol-3-phosphate dehydrogenase (GlpD) is an essential membrane enzyme, functioning at the central junction of respiration, glycolysis, and phospholipid biosynthesis. Its critical role is indicated by the multitiered regulatory mechanisms that stringently controls its expression and function. Once expressed, GlpD activity
Structural studies on nicotinamide adenine dinucleotide-linked L-glycerol 3-phosphate dehydrogenase crystallized from rat skeletal muscle.
T P Fondy et al.
The Journal of biological chemistry, 243(11), 3148-3160 (1968-06-10)
Optimal conditions for biomass and recombinant glycerol kinase production using the yeast Pichia pastoris
Aizemberg R, et al.
Food Technology and Biotechnology, 49(3), 329-329 (2011)
Susan K Boehlein et al.
The Plant journal : for cell and molecular biology, 99(1), 23-40 (2019-02-13)
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration of dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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