Anti-Gα _i3 antibody produced in rabbit

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.

Recognizes Gα_i3 and Gα₀ G-proteins.

synthetic peptide corresponding to the C-terminal region of Gα_i3.

Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.

Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca⁺² mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.

Solution of whole antiserum diluted in PBS, pH 7.4.

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