跳转至内容

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

For important updates on recent policy changes, please click here for more details.

Merck
CN
所有图片(1)

主要文件

查看所有文档

G2150

Sigma-Aldrich

L -谷氨酰胺 溶液

200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

别名:

L-Glutamine for cell culture

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
56-85-9
MDL编号:
MFCD00008044
UNSPSC代码:
12352209
eCl@ss:
32160406
PubChem化学物质编号:
24895088
NACRES:
NA.75
价格与库存信息目前不能提供

等级

Hybri-Max™

质量水平

300

无菌性

sterile-filtered

方案

≥99% (TLC)

表单

solution

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

颜色

clear colorless

运输

dry ice

储存温度

−20°C

正在寻找类似产品? 访问 产品对比指南

相关类别

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
G7513G854076523
L-Glutamine solution 200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

G2150

L-Glutamine solution

L-Glutamine solution 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

G7513

L-Glutamine solution

L-Glutamine meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

G8540

L-Glutamine

L-Glutamine certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

76523

L-Glutamine

form

solution

form

solution

form

powder

form

-

assay

≥99% (TLC)

assay

-

assay

99.0-101.0%

assay

-

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

-

color

clear colorless

color

-

color

white

color

white

一般描述

L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉和冷冻溶液中非常稳定。然而，在液体培养基或储备溶液中，L-谷氨酰胺的降解速度相对更快。为获得最佳细胞性能，通常需要在使用培养基前补充L-谷氨酰胺。

应用

L-谷氨酰胺溶液已用作罗斯威尔公园纪念研究所（RPMI）-1640培养基的补充剂，以培养人白血病单核细胞系用于细胞内分析和脾细胞用于CD4+ T细胞的体外激活。它还用作杂交瘤培养基的补充剂，用于抗原结合和杂交瘤克隆的初级筛选。

制备说明

使用细胞培养级水进行制备。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    RNBL6711
    RNBL0133
    RNBJ4044
    RNBJ0216
    RNBH7380

    没有发现合适的版本？

    如果您需要特殊版本，可通过批号或批次号查找具体证书。

    搜索COA

    已有该产品？

    在文件库中查找您最近购买产品的文档。

    访问文档库

    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.

      Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.

    6. Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?

      L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled.  After thawing, it will be an opalescent solution.  If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization.  To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed.  This will expose the product to the minimal amount of warming cycles.  

    7. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Production of human antibodies from transgenic mice.
    C Geoffrey Davis et al.
    Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
    Marina K Balabekova et al.
    Drug and chemical toxicology, 44(3), 277-285 (2019-03-09)
    Heavy metal pollution is rapidly increasing in the environment. It has been shown that exposure to vanadium and chromium is able to alter the immune response. Nevertheless, the mechanisms by which these metal pollutants mediate their immunomodulatory effects are not
    Abraham L Moure et al.
    Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
    Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
    Goonho Park et al.
    Cell reports, 31(13), 107839-107839 (2020-07-02)
    Amyloid β-protein (Aβ) toxicity is hypothesized to play a seminal role in Alzheimer's disease (AD) pathogenesis. However, it remains unclear how Aβ causes synaptic dysfunction and synapse loss. We hypothesize that one mechanism of Aβ-induced synaptic injury is related to
    [Intensive care: does administration of glutamine and antioxidants make sense? Supplementation of glutamine only in special cases].
    M Hecker et al.
    Deutsche medizinische Wochenschrift (1946), 138(25-26), 1344-1344 (2013-06-14)
    查看所有相关文献

    商品

    L-谷氨酰胺在细胞培养中的应用

    L-谷氨酰胺在杂交瘤细胞和哺乳动物细胞培养中的重要性和应用

    L-Glutamine in Cell Culture

    Importance and uses of glutamine in hybridoma and mammalian cell culture

    Chromatograms

    application for HPLC

    我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

    联系客户支持