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一般描述
β-葡萄糖醛酸酶（罗马蜗牛来源H-1型）是来源于罗马蜗牛的部分纯化粉末。来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
应用
生化/生理作用
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
质量
来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
单位定义
在pH 5.0、温度37°C条件下，一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞（30分钟实验）。
硫酸酯酶单元定义：一单位的硫酸酯酶每小时，pH5.0，37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
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商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
实验方案
酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
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