用途
kit sufficient for 50 reactions
质量水平
分析物化学类别
proteins (FLAG® Affinity Gels)
技术
immunoprecipitation (IP): suitable
运输
wet ice
储存温度
−20°C
一般描述
Sigma的FLAG免疫沉淀试剂盒可快速、高效地免疫沉淀和洗脱FLAG标记蛋白。免疫沉淀过程使用ANTI-FLAG M2亲和凝胶进行，它是一种与琼脂糖树脂共价连接的高特异性单克隆抗体。亲和树脂可使FLAG标记蛋白的结合高效进行，而无需预备步骤或校准。免疫沉淀后的FLAG标记蛋白可以在酸性条件下或使用竞争性FLAG肽，从树脂上高效地洗脱下来。免疫沉淀后的蛋白可用于电泳或其它活性检测方法，以检测它们的大小、翻译后修饰及相互作用。
应用
更多产品信息，请访问我们的FLAG®应用页面。
特点和优势
- 使用高特异性ANTI-FLAG M2亲和凝胶
- 无预备步骤或校准
- 3X FLAG™肽通过直接竞争实现高效和温和的洗脱
其他说明
所有步骤在2-8°C进行。
法律信息
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Eye Irrit. 2
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
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