usage
kit sufficient for 50 reactions
analyte chemical class(es)
proteins (FLAG® Affinity Gels)
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
更多产品信息，请访问我们的FLAG®应用页面。
Features and Benefits
- 利用高特异性的抗-FLAG M2亲和凝胶
- 无需预备步骤和校准
- 3xFLAG™肽通过直接竞争法实现高效温和的洗脱
General description
Sigma的FLAG免疫沉淀试剂盒可快速、高效地免疫沉淀和洗脱FLAG标记蛋白。免疫沉淀过程使用ANTI-FLAG M2亲和凝胶进行，它是一种与琼脂糖树脂共价连接的高特异性单克隆抗体。亲和树脂可使FLAG标记蛋白的结合高效进行，而无需预备步骤或校准。免疫沉淀后的FLAG标记蛋白可以在酸性条件下或使用竞争性FLAG肽，从树脂上高效地洗脱下来。免疫沉淀后的蛋白可用于电泳或其它活性检测方法，以检测它们的大小、翻译后修饰及相互作用。
Other Notes
所有步骤在2-8°C进行。
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
常规特殊物品
此项目有
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Kalpana Kannan et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), E1272-E1277 (2015-03-04)
High-grade serous ovarian cancer (HGSC) is among the most lethal forms of cancer in women. Excessive genomic rearrangements, which are expected to create fusion oncogenes, are the hallmark of this cancer. Here we report a cancer-specific gene fusion between BCAM
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Molecular therapy : the journal of the American Society of Gene Therapy, 28(3), 901-913 (2020-01-29)
Esophageal squamous cell carcinoma (ESCC) is a predominant cancer type in developing countries such as China, where ESCC accounts for approximately 90% of esophageal malignancies. Lacking effective and targeted therapy contributes to the poor 5-year survival rate. Recent studies showed
Mateusz Wydro et al.
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