推荐产品
生物来源
mouse
质量水平
偶联物
biotin conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous glycerol solution
种属反应性
all
浓度
~1 mg/mL
技术
dot blot: suitable (chemiluminescent detection)
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
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一般描述
FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。
应用
生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶(如链霉亲和素-过氧化物酶)的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时,首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站,了解更多产品详情。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站,了解更多产品详情。
外形
溶于50%甘油,10 mM磷酸钠,pH 7.25,含有150mM NaCl和0.02%叠氮化钠
制备说明
用TBS(0.05M Tris,pH7.4,含有0.15M NaCl)稀释抗体至终浓度为1-10μg/mL。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种:
分析证书(COA)
Nature communications, 4, 1505-1505 (2013-02-21)
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(6), 2025-2038 (2010-02-12)
To assess the effects and mechanisms of a CD200R1 agonist administered during the progressive stage of a multiple sclerosis model, we administered CD200R1 agonist (CD200Fc) or control IgG2a during the chronic phase of disease (days 10-30) in mice with experimental
Scientific reports, 7(1), 2071-2071 (2017-05-20)
Single-molecule studies of protein-nucleic acid interactions frequently require site-specific modification of long DNA substrates. The bacteriophage λ is a convenient source of high quality long (48.5 kb) DNA. However, introducing specific sequences, tertiary structures, and chemical modifications into λ-DNA remains technically
PloS one, 6(7), e22879-e22879 (2011-08-11)
Cyclin A2 is essential at two critical points in the somatic cell cycle: during S phase, when it activates CDK2, and during the G2 to M transition when it activates CDK1. Based on the crystal structure of Cyclin A2 in
Scientific reports, 4, 6391-6391 (2014-09-30)
The ends of eukaryotic chromosomes are capped by telomeres which consist of tandem G-rich DNA repeats stabilized by the shelterin protein complex. Telomeres shorten progressively in most normal cells due to the end replication problem. In more than 85% of
商品
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时,它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因,它们在细胞表面的表现通常被称为“糖”。
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
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