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Merck
CN

F9291

Sigma-Aldrich

抗FLAG® BioM2单克隆抗体 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.32

生物来源

mouse

质量水平

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

表单

buffered aqueous glycerol solution

种属反应性

all

浓度

~1 mg/mL

技术

dot blot: suitable (chemiluminescent detection)

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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一般描述

FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。

应用

生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶(如链霉亲和素-过氧化物酶)的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时,首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。

请访问我们的 FLAG®应用 门户网站,了解更多产品详情。

外形

溶于50%甘油,10 mM磷酸钠,pH 7.25,含有150mM NaCl和0.02%叠氮化钠

制备说明

用TBS(0.05M Tris,pH7.4,含有0.15M NaCl)稀释抗体至终浓度为1-10μg/mL。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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分析证书(COA)

Lot/Batch Number

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访问文档库

Hong-Won Lee et al.
Nature communications, 4, 1505-1505 (2013-02-21)
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
Yingru Liu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(6), 2025-2038 (2010-02-12)
To assess the effects and mechanisms of a CD200R1 agonist administered during the progressive stage of a multiple sclerosis model, we administered CD200R1 agonist (CD200Fc) or control IgG2a during the chronic phase of disease (days 10-30) in mice with experimental
Yoori Kim et al.
Scientific reports, 7(1), 2071-2071 (2017-05-20)
Single-molecule studies of protein-nucleic acid interactions frequently require site-specific modification of long DNA substrates. The bacteriophage λ is a convenient source of high quality long (48.5 kb) DNA. However, introducing specific sequences, tertiary structures, and chemical modifications into λ-DNA remains technically
Nawal Bendris et al.
PloS one, 6(7), e22879-e22879 (2011-08-11)
Cyclin A2 is essential at two critical points in the somatic cell cycle: during S phase, when it activates CDK2, and during the G2 to M transition when it activates CDK1. Based on the crystal structure of Cyclin A2 in
Helen Hwang et al.
Scientific reports, 4, 6391-6391 (2014-09-30)
The ends of eukaryotic chromosomes are capped by telomeres which consist of tandem G-rich DNA repeats stabilized by the shelterin protein complex. Telomeres shorten progressively in most normal cells due to the end replication problem. In more than 85% of

商品

蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时,它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因,它们在细胞表面的表现通常被称为“糖”。

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

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