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Merck
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安全信息

F9291

Sigma-Aldrich

抗FLAG® BioM2单克隆抗体 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

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别名:
单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk
UNSPSC代码:
12352203
NACRES:
NA.32

生物来源

mouse

质量水平

200

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous glycerol solution

种属反应性

all

浓度

~1 mg/mL

技术

dot blot: suitable (chemiluminescent detection)

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。

应用

生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶（如链霉亲和素-过氧化物酶）的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时，首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。

请访问我们的 FLAG®应用 门户网站，了解更多产品详情。

外形

溶于50％甘油，10 mM磷酸钠，pH 7.25，含有150mM NaCl和0.02％叠氮化钠

制备说明

用TBS（0.05M Tris，pH7.4，含有0.15M NaCl）稀释抗体至终浓度为1-10μg/mL。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Single-molecule studies of protein-nucleic acid interactions frequently require site-specific modification of long DNA substrates. The bacteriophage λ is a convenient source of high quality long (48.5 kb) DNA. However, introducing specific sequences, tertiary structures, and chemical modifications into λ-DNA remains technically
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Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
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商品

聚糖标记

蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时，它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因，它们在细胞表面的表现通常被称为“糖”。

Glycan Labeling

Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.

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