推荐产品
生物来源
mouse
偶联物
biotin conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous glycerol solution
种属反应性
all
浓度
~1 mg/mL
技术
dot blot: suitable (chemiluminescent detection)
同位素/亚型
IgG1
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一般描述
FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。
应用
生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶（如链霉亲和素-过氧化物酶）的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时，首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站，了解更多产品详情。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站，了解更多产品详情。
外形
溶于50％甘油，10 mM磷酸钠，pH 7.25，含有150mM NaCl和0.02％叠氮化钠
制备说明
用TBS（0.05M Tris，pH7.4，含有0.15M NaCl）稀释抗体至终浓度为1-10μg/mL。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Hong-Won Lee et al.
Nature communications, 4, 1505-1505 (2013-02-21)
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
Yingru Liu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(6), 2025-2038 (2010-02-12)
To assess the effects and mechanisms of a CD200R1 agonist administered during the progressive stage of a multiple sclerosis model, we administered CD200R1 agonist (CD200Fc) or control IgG2a during the chronic phase of disease (days 10-30) in mice with experimental
Nawal Bendris et al.
PloS one, 6(7), e22879-e22879 (2011-08-11)
Cyclin A2 is essential at two critical points in the somatic cell cycle: during S phase, when it activates CDK2, and during the G2 to M transition when it activates CDK1. Based on the crystal structure of Cyclin A2 in
Helen Hwang et al.
Scientific reports, 4, 6391-6391 (2014-09-30)
The ends of eukaryotic chromosomes are capped by telomeres which consist of tandem G-rich DNA repeats stabilized by the shelterin protein complex. Telomeres shorten progressively in most normal cells due to the end replication problem. In more than 85% of
Qing Xu et al.
Nature communications, 8(1), 425-425 (2017-09-06)
Tumor necrosis factor (TNF) has a critical role in diverse cellular events including inflammation, apoptosis and necroptosis through different signaling complexes. However, little is known about how the transition from inflammatory signaling to the engagement of death pathways is modulated.
商品
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时，它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因，它们在细胞表面的表现通常被称为“糖”。
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
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