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Merck
CN

F8646

Sigma-Aldrich

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

FITC conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

储存条件

protect from light

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.

特异性

Binds mouse IgG; does not bind other mouse Igs.

免疫原

Purified mouse IgG

应用

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.

其他说明

Antibody adsorbed with bovine, equine, and human serum proteins.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

制备说明

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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K A Muczynski et al.
Journal of immunology (Baltimore, Md. : 1950), 160(7), 3207-3216 (1998-04-08)
We compared HLA class II expression in a human melanoma line (a nonprofessional APC), induced by IFN-gamma or by stable transfection with CIITA, with constitutive class II expression in an EBV-transformed B lymphoblastoid cell line (a professional APC) from the
J Gysin et al.
Infection and immunity, 67(12), 6596-6602 (1999-11-24)
We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and
M Coulpier et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 16(19), 5897-5904 (1996-10-01)
The molecular events leading to motoneuronal death are still poorly understood. In mammals, the bcl-2 proto-oncogene, which encodes a membrane-associated protein, has been shown to suppress both developmental motoneuronal death and experimental axotomy-induced motoneuronal death. We assessed a potential protective
Daniel Sehic et al.
American journal of clinical pathology, 141(6), 828-833 (2014-05-20)
Successful further treatment of Wilms tumors (WTs) after preoperative chemotherapy and surgery depends on correct histopathologic risk stratification, including quantification of remaining blastemal elements. In the present study, we assessed the usefulness of protein markers for the detection of WT
Gabrielle Dias Salton et al.
Cancer biology & therapy, 18(8), 560-570 (2017-07-12)
Eukaryote initiation factor 2 subunit β (eIF2β) plays a crucial role in regulation protein synthesis, which mediates the interaction of eIF2 with mRNA. eIF2β contains evolutionarily conserved polylysine stretches in amino-terminal region and a zinc finger motif in the carboxy-terminus.

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