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无菌性
γ-irradiated
产品线
BioXtra
形式
powder
分子量
48.4 kDa
技术
cell culture | mammalian: suitable
溶解性
water: 1 mg/mL, clear to slightly hazy, colorless to faintly yellow
UniProt登记号
储存温度
2-8°C
基因信息
bovine ... FETUB(504615)
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一般描述
Fetuin is a protein component of serum which contributes to the attachment and spreading of cells in culture medium. Researchers often add serum for this purpose. Fetuin has been used at a concentration of 500 μg/ml to supplement serum-free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells. Fetuin can be used as a glycoprotein standard for carbohydrate structure in a glycoprotein. The structural elucidation of the carbohydrate portion of fetuin has been published. The cDNA and amino acid sequences has been reported. Fetuin contains 12 Cys residues, all involved in disulfide bridges.
The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.
The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.
分析说明
This product is cell culture tested (0.5 g/L) and is appropriate for use in cell culture applications.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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Ultracentrifugal and electrophoretic studies on fetuin.
K O PEDERSEN
The Journal of physical and colloid chemistry, 51(1), 164-171 (1947-01-01)
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