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NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
GT-34, monoclonal
Application:
dot immunobinding
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
particle immunofluorescence
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
particle immunofluorescence
Species reactivity:
sheep, goat, bovine
Citations:
9
Technique(s):
dot immunobinding: 1:32
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
particle immunofluorescence: 1:32
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
particle immunofluorescence: 1:32
产品名称
单克隆抗 山羊/绵羊 IgG, clone GT-34, purified from hybridoma cell culture
biological source
mouse
conjugate
FITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
secondary antibodies
clone
GT-34, monoclonal
form
buffered aqueous solution
species reactivity
sheep, goat, bovine
should not react with
rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
storage condition
protect from light
technique(s)
dot immunobinding: 1:32
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
particle immunofluorescence: 1:32
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
抗体与位于山羊 IgG1 和 IgG2 重链上的表位反应。
Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
Application
Biofile 39 Navya load
Biochem/physiol Actions
IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization. Goat antibodies against many analytes are widely used in research as primary antibodies in various assay techniques. Second antibodies are that used to detect goat antibodies may lack specificity for goat immunoglobulins, and in many instances will also recognize other immunoglobulins that appear in the preparation being tested. This results in the need for extensive adsorption of the second antibody. Therefore, using a monoclonal antibody to goat IgG that is devoid of a binding capacity to other species can serve as a useful tool in many applications.
Monoclonal Anti-Goat IgG, FITC Conjugate is immunospecific for goat IgG. The antibody does not react with human, mouse, rat, rabbit, guinea pig, horse, or chicken IgGs.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Physical form
Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide
Preparation Note
For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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