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Merck
CN

F4637

Sigma-Aldrich

Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

别名:

Rabbit Anti-Human Polyvalent Immunoglobulins (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

rabbit

质量水平

偶联物

FITC conjugate

抗体形式

IgG fraction of antiserum

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

储存条件

protect from light

技术

ANA-indirect immunofluorescence: 1:128
direct immunofluorescence: 1:80

储存温度

−20°C

靶向翻译后修饰

unmodified

一般描述

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Purified human IgA, IgG and IgM are used to develop anti-sera in rabbits. The IgG fraction of each anti-sera is purified and conjugated to Fluorescein Isothiocyanate (FITC), in an alkaline reaction. Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody specifically recognizes human IgA, IgG and IgM.

免疫原

Purified human IgA, IgG, and IgM

应用

Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a working antibody dilution of 1:128. For anti-nuclear antibody assay of acetone-fixed rat liver cells, a minimum dilution of 1:128 is recommended. For direct labeling of human tonsil sections, a dilution of 1:80 may be used. This antibody was used to stain C2C12 cell line derived from mouse myoblasts at a dilution of 1:100
Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit has been used in immunohistochemical analysis and immunofluorescence

生化/生理作用

IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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分析证书(COA)

Lot/Batch Number

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