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安全信息

F4512

Sigma-Aldrich

抗-人IgG（全分子）-FITC 兔抗

IgG fraction of antiserum, buffered aqueous solution

别名:

Rabbit Anti-Human IgG (whole molecule)−Fluorescein isothiocyanate

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1 EA
CN¥4,637.12

About This Item

MDL编号:
MFCD00162487
UNSPSC代码:
12352203
NACRES:
NA.46

CN¥4,637.12

请联系客服了解存货情况
获取大包装报价

生物来源

rabbit

质量水平

200

偶联物

FITC conjugate

抗体形式

IgG fraction of antiserum

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

储存条件

protect from light

技术

immunofluorescence: 1:64-1:128 using Hep2 cells

储存温度

−20°C

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F4637F5137F7512
Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

F4512

Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit

Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

F4637

Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit

Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

F5137

Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit

Anti-Rabbit IgG (whole molecule)−FITC antibody produced in sheep affinity isolated antibody, buffered aqueous solution

F7512

Anti-Rabbit IgG (whole molecule)−FITC antibody produced in sheep

technique(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

technique(s)

ANA-indirect immunofluorescence: 1:128, direct immunofluorescence: 1:80

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:100

technique(s)

direct immunofluorescence: 1:160, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

sheep

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

一般描述

IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。荧光染料染料缀合物FITC（异硫氰酸荧光素）可用于对细胞或组织等生物结构的抗原-抗体相互作用进行定位。用洗涤缓冲液以1:100稀释的抗人IgG（全分子）-FITC抗体可用于流式细胞术，以分析抗体亲和力和病毒结合。该抗体也可用于间接免疫荧光抗体试验。FITC标记的抗人IgG抗体与人特异性反应。
IgG抗体亚型是免疫系统中含量最高的血清免疫球蛋白。它由B细胞分泌，存在于血液和细胞外液中，可防止细菌、真菌和病毒引起的感染。母体IgG通过胎盘传递至胎儿，对新生儿的感染免疫防御能力至关重要。抗人IgG（全分子）-FITC抗体对人IgG具有特异性。兔抗人IgG在碱性反应中与结晶异硫氰酸荧光素（FITC）缀合，然后进一步纯化去除游离的FITC。

免疫原

IgG来自合并的正常人血清

应用

成功使用该抗体的应用以及相关的同行评审论文如下。
免疫荧光（1篇）
抗人IgG（全分子）-FITC抗体可在间接免疫荧光法中用作二抗，也可用于流式细胞术 。
该抗体可以1:64的工作稀释度用于人外周血淋巴细胞免疫荧光染色。在丙酮固定的小鼠肝细胞和抗核抗体阳性血清上用作第一抗体时，最少使用1:64的工作稀释度。在对NIH3T3细胞进行标记的流式细胞术中，抗体以1:100的工作稀释度使用。该抗体也用于人血清ELISA。

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

除非我们的产品目录或产品随附的其他公司文档中另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000435609
    0000435640
    0000435640
    0000435609
    0000388494

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