推荐产品
生物来源
mouse
质量水平
偶联物
FITC conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous solution
种属反应性
all
浓度
~1 mg/mL
技术
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
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一般描述
FLAG M2-FITC被用于检测Flag融合蛋白。该单克隆抗体来自于小鼠,并与异硫氰酸荧光素(FITC)共价结合。该抗体可识别N末端、Met N末端和C末端的FLAG序列。结合不依赖于钙。F4049是一种亲和纯化的抗体,在大多数应用中可提高灵敏度。
应用
抗体适用于免疫荧光、免疫细胞化学、免疫组织化学、免疫印迹、流式细胞术和电子显微镜分析。该抗体特别适用于检测在小鼠宿主中表达的Flag融合蛋白,其中二抗可能引起交叉反应。
请访问我们的 FLAG® 应用 门户网站,了解更多产品详情。
请访问我们的 FLAG® 应用 门户网站,了解更多产品详情。
外形
溶于10 mM磷酸盐缓冲盐水,含有1% BSA和0.1%叠氮化钠,pH 7.4
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种:
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Nature communications, 7, 13326-13326 (2016-11-05)
Translesion polymerase eta (polη) was characterized for its ability to replicate ultraviolet-induced DNA lesions that stall replicative polymerases, a process promoted by Rad18-dependent PCNA mono-ubiquitination. Recent findings have shown that polη also acts at intrinsically difficult to replicate sequences. However
Molecular pharmacology, 71(6), 1487-1493 (2007-03-01)
Multidrug and toxin extrusion 1 (MATE1) has been isolated as an H(+)/organic cation antiporter located at the renal brush-border membranes. Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H(+)/organic cation
商品
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时,它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因,它们在细胞表面的表现通常被称为“糖”。
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
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