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Merck
CN

F3761

Sigma-Aldrich

抗人 κ 轻链(结合和游离)−FITC 山羊抗

affinity isolated antibody, buffered aqueous solution

别名:

Goat Anti-Human Kappa Light Chain (Bound and Free)−Fluorescein isothiocyanate

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

FITC conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

形式

buffered aqueous solution

技术

direct immunofluorescence: 1:16

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... IGK@(50802)

相关类别

一般描述

哺乳动物抗体含有两种轻链中的一种,称为 κ 或 lamba。每个链包含一个常数和一个可变域。哺乳动物抗体含有两条 κ 或两条 λ 轻链。
异硫氰酸荧光素 (FITC) 是一种荧光素衍生物(荧光染料),用于标记抗体,包括二抗,用于基于荧光的测定和程序。FITC 激发波长为 495 nm,发射波长为 521 nm。
当对照纯化的结合 κ 轻链(人 IgA、IgG 和 IgM)和游离 κ 轻链(Bence Jonesκ 骨髓瘤)进行检测时,抗人 κ 轻链(结合和游离)−FITC 抗体对人 κ 轻链具有特异性蛋白)。结合物不与结合或游离 λ 轻链反应。
抗人 κ轻链(结合和游离)抗体获自山羊抗血清。免疫特异性纯化去除所有不与 κ轻链(结合和游离)。山羊抗 κ 抗体轻链与异硫氰酸荧光素 (FITC) 结合。抗体制备是针对人 κlight(人 IgA、IgG 和 IgM)和游离 κ轻链(Bence Jonesκ骨髓瘤蛋白)。结合物不与结合或游离 λ 反应轻链。

免疫原

本斯琼斯-κ骨髓瘤蛋白

应用

山羊多克隆抗人 κ 轻链(结合和游离)−FITC 抗体可用于通过荧光技术检测和定量含 κ 轻链的抗体级别。
成功使用该抗体的应用以及相关同行评审论文如下所示。
流式细胞术/细胞分选(1 篇)
抗人κ轻链(结合和游离)-FITC抗体可用于免疫荧光,工作抗体稀释度为1:16。该抗体用于各种研究中的流式细胞术分析。

外形

0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液,含 1% 牛血清白蛋白和 15 mM 叠氮化钠

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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Annalee W Nguyen et al.
Methods in molecular biology (Clifton, N.J.), 2070, 397-422 (2019-10-19)
While antibody libraries are traditionally screened in phage, bacterial, or yeast display formats, they are produced in large scale for pharmaceutical and commercial use in mammalian cell lines. The simpler organisms used for screening have significantly different folding and glycosylation
Steffen Dickopf et al.
Biological chemistry (2019-03-14)
A novel bispecific antibody format was applied to generate T cell-engaging antibodies. The TriFab format is a trivalent IgG-shaped entity composed of two Fab arms that bind to antigens on the surface of tumor cells, which are linked via flexible
Dieter Palmberger et al.
PloS one, 7(4), e34226-e34226 (2012-04-10)
Recombinant production of therapeutically active proteins has become a central focus of contemporary life science research. These proteins are often produced in mammalian cells, in order to obtain products with post-translational modifications similar to their natural counterparts. However, in cases
Frederick W Jacobsen et al.
Journal of immunology (Baltimore, Md. : 1950), 186(1), 341-349 (2010-12-07)
Studies for vaccine and human therapeutic Ab development in cynomolgus monkeys (cynos) are influenced by immune responses, with Ab responses playing a significant role in efficacy and immunogenicity. Understanding the nature of cyno humoral immune responses and characterizing the predominant
Soo Hean Gary Khoo et al.
Biotechnology and bioengineering, 102(1), 188-199 (2008-08-07)
The understanding of how cellular productivity is modulated in cell lines is of significant importance in the biopharmaceutical industry. Often, single molecular mechanisms fail to fully explain how specific antibody productivity is enhanced during proliferation arrest. Previously, we reported that

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