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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M1, monoclonal
形式
buffered aqueous solution
纯化方式
using Protein A
种属反应性
all
浓度
2-5 mg/mL
技术
western blot: 10 μg/mL
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
一般描述
抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。
纯化方法-蛋白A
纯化方法-蛋白A
免疫原
FLAG；肽序列 DYKDDDDK
应用
小鼠产生的单克隆抗-FLAG® M1 抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹或EIA 。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
请访问我们的FLAG® 应用门户网站，了解更多产品详情。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
请访问我们的FLAG® 应用门户网站，了解更多产品详情。
生化/生理作用
当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合，因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性；该复合物在不存在钙离子的情况下解离。
外形
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
制备说明
将抗体稀释至 10ug/mL，置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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