推荐产品
生物来源
(Bioreactor)
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M1, monoclonal
表单
buffered aqueous solution
纯化方式
using Protein A
种属反应性
all
浓度
2-5 mg/mL
技术
western blot: 10 μg/mL
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
一般描述
抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。
纯化方法-蛋白A
纯化方法-蛋白A
免疫原
FLAG；肽序列 DYKDDDDK
应用
小鼠产生的单克隆抗-FLAG® M1 抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹或EIA 。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
请访问我们的FLAG® 应用门户网站，了解更多产品详情。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
请访问我们的FLAG® 应用门户网站，了解更多产品详情。
生化/生理作用
当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合，因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性；该复合物在不存在钙离子的情况下解离。
外形
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
制备说明
将抗体稀释至 10ug/mL，置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。
其他说明
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Mohammad Al Sorkhy et al.
BMC cancer, 12, 45-45 (2012-01-28)
Spy1 is a novel 'cyclin-like' activator of the G1/S transition capable of enhancing cell proliferation as well as inhibiting apoptosis. Spy1 protein levels are tightly regulated during normal mammary development and forced overexpression in mammary mouse models accelerates mammary tumorigenesis.
Andrew Best et al.
Nature communications, 5, 4760-4760 (2014-09-12)
Alternative splicing--the production of multiple messenger RNA isoforms from a single gene--is regulated in part by RNA binding proteins. While the RBPs transformer2 alpha (Tra2α) and Tra2β have both been implicated in the regulation of alternative splicing, their relative contributions
Min Luo et al.
Cell research, 20(2), 211-222 (2010-01-27)
As a critical apoptosis executioner, caspase-3 becomes activated and then enters into the nucleus to exert its function. However, the molecular mechanism of this nuclear entry of active caspase-3 is still unknown. In this study, we revealed that caspase-3 harbors
Chandrahasa R Yellaturu et al.
The Journal of biological chemistry, 284(46), 31726-31734 (2009-09-18)
The regulation of lipid homeostasis by insulin is mediated in part by the enhanced transcription of the gene encoding sterol regulatory element-binding protein-1c (SREBP-1c). The nascent SREBP-1c is embedded in the endoplasmic reticulum (ER) and must be transported to the
Chandrahasa R Yellaturu et al.
The Journal of biological chemistry, 284(12), 7518-7532 (2009-01-23)
The regulation of lipid homeostasis by insulin is mediated in part by the enhanced transcription of the gene encoding SREBP-1c (sterol regulatory element-binding protein-1c). Nascent SREBP-1c is synthesized and embedded in the endoplasmic reticulum (ER) and must be transported to
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门