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安全信息

F2555

Sigma-Aldrich

单克隆抗-FLAG®

clone SIG1-25, ascites fluid

别名:

抗-ddddk, 抗-dykddddk

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.32

生物来源

rabbit

质量水平

200

偶联物

unconjugated

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

SIG1-25, monoclonal

技术

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein

同位素/亚型

IgG

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

相关类别

一般描述

表位标签提供的方法可定位各种类型细胞中的基因产物、研究蛋白和蛋白复合物的拓扑结构、识别相关蛋白，并在没有蛋白特异性抗体时对新鉴定、丰度低或免疫原性低的蛋白进行表征。FLAG® 肽序列标签可以在N-端、前面有甲硫氨酸残基的N-端、C-端或在靶蛋白的内部位置进行。FLAG也可以与其他标签一起使用。短FLAG标签或序列及其高亲水性倾向于降低干扰蛋白表达、蛋白水解成熟、抗原性和功能的可能性。N-端FLAG® 肽序列含有独特的肠激酶切割位点，使其可以从纯化的融合蛋白中完全去除。已有报道，C-端FLAG® 肽的Cu2+离子，可从融合蛋白催化切割。在大鼠和小鼠Mg2+依赖性蛋白b-磷酸酶以及人类细胞和牛细胞酶中发现了一种序列模体，其八个氨基酸残基中有五个与FLAG®肽相同。兔抗-FLAG®抗体来自SIG1-25杂交瘤细胞。杂交瘤细胞是通过接种偶联KLH的FLAG®肽链序列的兔骨髓瘤细胞和脾细胞融合获得。

特异性

这种单克隆抗体可识别FLAG序列N端

免疫原

FLAG肽链序列DYKDDDK

应用

ANTI-FLAG®单克隆抗体（兔抗）适用：

  • 免疫荧光
  • 蛋白质印迹分析
  • 免疫细胞化学
  • 间接ELISA

更多产品信息，请访问我们的FLAG®应用页面。

外形

以含有15mM叠氮化钠（作为防腐剂）的腹水形式提供。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000368976
0000223737
0000223737
0000193746
0000165267

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Why should I use Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit?

    The main application for Product F2555 rabbit monoclonal is for immunoblotting after immunoprecipitation with M2, which is a mouse antibody. This enables using a secondary antibody (anti-rabbit) that will not bind the M2 mouse heavy (50 kD) and light chains (25 kD).

  6. Will Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25, recognize an internal FLAG tag protein?

    F2555 will recognize N-terminal and C-terminal FLAG fusion proteins.

  7. What positive controls can be used for immunoblotting with Product F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25?

    Two of the FLAG-tagged proteins offered by Sigma-Aldrich are the Amino-terminal FLAG-BAP fusion protein (Product No. P7582) and the Carboxy-terminal FLAG-BAP fusion protein (Product No. P7457). Both of these proteins should work with the F2555 rabbit ANTI-FLAG.

  8. Is Product F2555 a monoclonal or polyclonal FLAG® antibody?

    F2555 is a monoclonal antibody, raised in rabbits. [Note: Nearly all of the monoclonal antibodies sold by Sigma-Aldrich are raised in mice.]

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Qungang Qi et al.
The Journal of biological chemistry, 287(37), 31482-31493 (2012-07-18)
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eLife, 6 (2017-07-01)
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Hamajima N, et al.
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