产品名称
单克隆抗-FLAG®, clone SIG1-25, ascites fluid
biological source
rabbit
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SIG1-25, monoclonal
technique(s)
immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
isotype
IgG
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
Biochem/physiol Actions
General description
Immunogen
Physical form
Legal Information
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why should I use Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit?
The main application for Product F2555 rabbit monoclonal is for immunoblotting after immunoprecipitation with M2, which is a mouse antibody. This enables using a secondary antibody (anti-rabbit) that will not bind the M2 mouse heavy (50 kD) and light chains (25 kD).
Will Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25, recognize an internal FLAG tag protein?
F2555 will recognize N-terminal and C-terminal FLAG fusion proteins.
What positive controls can be used for immunoblotting with Product F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25?
Two of the FLAG-tagged proteins offered by Sigma-Aldrich are the Amino-terminal FLAG-BAP fusion protein (Product No. P7582) and the Carboxy-terminal FLAG-BAP fusion protein (Product No. P7457). Both of these proteins should work with the F2555 rabbit ANTI-FLAG.
Is Product F2555 a monoclonal or polyclonal FLAG® antibody?
F2555 is a monoclonal antibody, raised in rabbits. [Note: Nearly all of the monoclonal antibodies sold by Sigma-Aldrich are raised in mice.]
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