生物来源
synthetic
质量水平
检测方案
≥70% (HPLC)
形式
powder
杂质
≤0.3% Riboflavin
溶解性
water: 50 mg/mL
储存温度
−20°C
SMILES字符串
O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C
InChI
1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1
InChI key
BHRVCJBIICJWTH-APQIITSESA-M
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应用
核黄素5′-单磷酸钠盐水合物用于:
- 作为测定缓冲液成分,确定乳酸菌( L. lactis )细胞的发光情况
- 作为反应混合物成分,用于一氧化氮合酶(NOS)酶活性测定
- 高效液相色谱(HPLC),分析黄素单核苷酸(FMN)环化酶产物
- 萤火虫荧光素酶的荧光素酶测定
生化/生理作用
核黄素5′-单磷酸(FMN)是一种核黄素衍生物,主要用作氧化还原反应1到2个电子转移的辅基。它是还原型烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶、一氧化氮合酶和氨三乙酸单加氧酶等酶的辅因子。核黄素5′-单磷酸可用作底物,研究FMN磷酸水解酶的特异性和动力学特性,也可以通过核糖开关研究基于分子感应的基因调控。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Luciferase detection during stationary phase in Lactococcus lactis
Applied and Environmental Microbiology, 73(14), 4704-4706 (2007)
European journal of biochemistry, 249(3), 777-785 (1997-12-12)
In order to gain some insight into mitochondrial flavin biochemistry, rat liver mitochondria essentially free of lysosomal and microsomal contamination were prepared and their capability to metabolise externally added and endogenous FAD and FMN tested both spectroscopically and via HPLC.
Establishment of patterned thalamocortical connections does not require nitric oxide synthase
The Journal of Neuroscience, 18(21), 8826-8838 (1998)
Enzymic formation of riboflavin 4?, 5?-cyclic phosphate from FAD: evidence for a specific low-Km FMN cyclase in rat liver1
The Biochemical Journal, 330(2), 881-888 (1998)
The Journal of organic chemistry, 72(17), 6329-6342 (2007-06-22)
Flavin coenzymes play a variety of roles in biological systems. This Perspective highlights the chemical versatility of flavins by reviewing research on five flavoenzymes that have been studied in our laboratory. Each of the enzymes discussed in this review [the
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