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Merck
CN

F2168

抗-α-微管蛋白−FITC抗体，小鼠单克隆抗体

clone DM1A, purified from hybridoma cell culture

别名:

单克隆抗-α-Tubulin

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203

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产品名称

抗-α-微管蛋白−FITC抗体，小鼠单克隆抗体, clone DM1A, purified from hybridoma cell culture

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DM1A, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:50 using cultured BHK cells

isotype

IgG1

UniProt accession no.

P68366

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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Application

单克隆抗-α-微管蛋白抗体适用于以下免疫染色：
  • 呼吸道上皮组织，用于研究确定小鼠纤毛中微管蛋白表达
  • 乳腺癌组织切片，研究LMO4对中心体扩增和有丝分裂纺锤体异常的影响
  • 卵母细胞的纺锤体和染色体
小鼠单克隆抗体用于：
  • 通过免疫荧光和共聚焦显微镜检测肺癌细胞中的微管蛋白
  • 人体胚胎微管和脾淋巴母细胞有丝分裂纺锤体微管的免疫荧光染色。

Biochem/physiol Actions

α−微管蛋白N-末端结构域以非交换机制与GTP结合，而高度酸性的C-末端结构域则能够结合微管相关蛋白（MAP）。这两个结构域都与微管自组装相关。微管在有丝分裂、细胞内转运、睫状鞭毛运动以及细胞形状的产生和维持中起到了结构性和活动元素的作用。微管蛋白 α-4A基因突变会导致称作肌萎缩侧索硬化的神经退行性疾病。
该抗体在免疫印迹检测中对α-tubulin具有特异性，并可用于培养细胞或组织切片中α-tubulin的定位。该抗体可与鸡成纤维细胞产生最佳的反应。
该抗体在免疫印迹试验中对 α-微管蛋白具有特异性，可用于培养细胞或组织切片中定位 α-微管蛋白。抗体与鸡成纤维细胞反应最佳。小鼠单克隆抗-α-微管蛋白FITC抗体可特异性识别 α-微管蛋白羧基末端部分的表位。它可对人、猴、牛、鸡、山羊、鼠、大鼠、沙鼠、仓鼠、大鼠袋鼠、两栖动物、海胆、锥虫、酵母、真菌和烟草中的 α-微管蛋白进行定位。

Disclaimer

除非我们的产品目录或产品随附的其他公司文档中另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。

General description

αβ-微管蛋白是微管的主要建构单元，属于微管蛋白超家族。它们在所有真核细胞中普遍存在。它在许多生物体中存在多种同种型。†它是一个异二聚体，具有细胞内中空圆柱形丝状结构。它定位于人类染色体2q35。 α/β-微管蛋白和 γ-微管蛋白是蛋白微管蛋白超家族成员。α/β-微管蛋白是异二聚体，由一条 α-微管蛋白链和一条 β-微管蛋白链组成；每个亚基的分子量均为55 kDa，具有相当大的同源性。微管蛋白是一种高度保守的蛋白质，具有两个末端结构域：大N末端和C末端。
抗α-微管蛋白(鼠IgG1亚型)单克隆抗体由DM1A 杂交瘤产生，这一杂交瘤由小鼠骨髓瘤细胞和免疫BALB/c小鼠的脾细胞融合产生。纯化的鸡脑微管可用作免疫原。通过使用小鼠单克隆抗体同种型试剂（产品号ISO2）和双扩散免疫检测，以确定同种型。产物是蛋白A纯化的与异硫氰酸荧光素缀合的单克隆抗-α-微管蛋白抗体异构体I。由凝胶过滤纯化，不含可检测出的游离FITC。

Immunogen

来自鸡胚脑的微管。

Physical form

溶于含有1％牛血清白蛋白和15mM叠氮化钠的0.01M磷酸盐缓冲盐水（pH7.4）中

Preparation Note

如需连续使用，可在2-8 °C保存至多一月。对于长期存储，请等分并冷冻保存。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存后发生轻微浑浊，请在使用前通过离心澄清溶液。

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000508938
0000508938
0000461117
0000461117
0000453508

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the immunogen for Monoclonal Anti-α-Tubulin antibody, clone DM1A?

    The immunogen for Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A is microtubules from chicken embryo brain.  This antibody recognizes an epitope located at the C-terminal end of the α-tubulin isoform (amino acids 426-430) as noted in the following references:Breitling, F., and Little, M., J. Molec. Biol., 189, 367-370 (1986).Wolff, A., et al., Biol. Cell, 63, 319-326 (1988).Serrano, L., et al., Anal. Biochem., 159, 253-259 (1986).

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    Ask a Scientist here.

A revised nomenclature for the human and rodent a-tubulin gene family.
Khodiyar V K, et al.
Genomics, 90(2), 285-289 (2007)
Lingyan Wang et al.
Bioscience reports, 29(5), 315-320 (2008-11-05)
The golden hamster is an attractive model organism for studying reproductive physiology, oncology, genetics and virology. In an effort to establish experimental protocols necessary for cloning golden hamsters, we examined changes in the reciprocal position of the FPB (first polar
Downregulation of survivin and aurora A by histone deacetylase and RAS inhibitors: a new drug combination for cancer therapy.
Biran A, et al.
International Journal of Cancer. Journal International Du Cancer, 128(3), 691-701 (2011)
Y F Inclán et al.
Journal of cell science, 114(Pt 2), 413-422 (2001-01-10)
alphabeta-tubulin heterodimers self-assemble to form microtubules nucleated by gamma-tubulin in the cell. Gamma-tubulin is believed to recruit the alphabeta-tubulin dimers that form the minus ends of microtubules, but the molecular mechanism of this action remains a matter of heated controversy.
Yong-Han Kim et al.
Molecular reproduction and development, 86(8), 972-983 (2019-05-29)
Mammalian oocytes lack centrioles but can generate bipolar spindles using several different mechanisms. For example, mouse oocytes have acentriolar microtubule organization centers (MTOCs) that contain many components of the centrosome, and which initiate microtubule polymerization. On the contrary, human oocytes
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