推荐产品
生物来源
rabbit
质量水平
偶联物
FITC conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
储存条件
protect from light
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
indirect immunofluorescence: 1:320
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.
特异性
Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.
免疫原
Purified rat IgG
应用
Anti-Rat IgG (whole molecule)-FITC antibody has been used:
- in immunolabelling for confocal laser scanning microscopy (CLSM)
- in immunostaining
Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.
生化/生理作用
IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
其他说明
Antibody adsorbed with human IgG.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
制备说明
Adsorbed to reduce background staining with human samples.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Structural network of arabinogalactan proteins (AGPs) and pectins in apple fruit during ripening and senescence processes
Leszczuk A, et al.
Plant Science, 275, 36-48 (2018)
Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage
Zhou Y, et al.
Experimental and Therapeutic Medicine, 12(4), 2671-2680 (2016)
C H Yeung et al.
Molecular human reproduction, 7(7), 617-624 (2001-06-23)
HE5 (CD52) is a glycoprotein which is secreted by the epididymis and which becomes inserted onto maturing spermatozoa. We have previously shown that, in cynomolgus monkey spermatozoa, changes occur upon maturation rendering cryptic the epitope to the monoclonal antibody CAMPATH-1G;
Dhika Amanda et al.
Plant physiology, 172(4), 2204-2218 (2016-10-21)
The plant epidermis is crucial to survival, regulating interactions with the environment and controlling plant growth. The phytocalpain DEFECTIVE KERNEL1 (DEK1) is a master regulator of epidermal differentiation and maintenance, acting upstream of epidermis-specific transcription factors, and is required for
Sílvia Coimbra et al.
Journal of experimental botany, 58(15-16), 4027-4035 (2007-11-28)
Some of the most important changes that occur in plants during sexual reproduction involve the transition from a sporophytic to a gametophytic type of development. In this paper, these changes were evaluated for Arabidopsis thaliana. The results obtained clearly show
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