重组
expressed in E. coli
质量水平
检测方案
≥95% (SDS-PAGE)
形式
lyophilized powder
包装
pkg of 1 kit (3 components)
应用
CRISPR
运输
ambient
储存温度
−20°C
一般描述
来自化脓链球菌的重组eSpCas9蛋白（~160 KD）是一种可用于基因组工程改造实验的即用型试剂。当与靶标特异性的向导RNA结合时，eSpCas9可作为靶向核酸酶，用于细胞培养物的转染和通过单细胞胚胎注射加速基因修饰动物的发育。
应用
功能基因组学/靶标验证/基因组编辑
特点和优势
- 与野生型Cas9相比具有增强的特异性
- 高活性
- 可立即用于注射/转染
包装
50 μg 装
250 μg 装
250 μg 装
组分
每个试剂盒包含：
- 一瓶eSpCas9重组蛋白
- 一瓶1×稀释缓冲液，1 mL
- 一瓶含有甘油的1 mL无核酸酶水
原理
新型工程化的eSpCas9可对已经建立的CRISPR系统进行有效的靶向基因编辑，并具有更低脱靶效应的优势。 如Slaymaker等人所述，SpCas9染色体结合基序中的点突变提供了更高的中靶保真度，且不会导致切割效率的损失。
重悬
冻干的化脓性链球菌eSpCas9蛋白应重悬于所提供的重构溶液中，以获得相应浓度。轻轻敲打试管以完全溶解冻干的粉末，在冰上孵育10分钟，然后离心试管以使内容物离心至试管底部。
其他说明
仅试剂盒组分
产品编号
说明
- Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli
- Dilution buffer for Cas9 proteins
- Reconstitution solution for Cas9 proteins
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Evan R Stark-Dykema et al.
Scientific reports, 12(1), 8554-8554 (2022-05-21)
Mammalian sex chromosomes are enriched for large, nearly-identical, palindromic sequences harboring genes expressed predominately in testicular germ cells. Discerning if individual palindrome-associated gene families are essential for male reproduction is difficult due to challenges in disrupting all copies of a
CRISPR off-target analysis in genetically engineered rats and mice
Keith R. Anderson, et al
Nature Methods (2018)
CRISPR/Cas9 Endonuclease-Mediated Mouse Genome Editing of One-Cell and/or Two-Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Interhomolog Repair Mechanism.
Garza, et al.
Methods in Molecular Biology, 2631, 253-266 (2023)
Vasin Dumrongprechachan et al.
eLife, 11 (2022-10-15)
Mammalian axonal development begins in embryonic stages and continues postnatally. After birth, axonal proteomic landscape changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Comprehensive profiling of axonal proteomes across neurodevelopment is limited, with most studies lacking cell-type and
Yuki Matsushita et al.
Nature communications, 14(1), 2383-2383 (2023-05-15)
The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. It is well-described that leptin receptor (LepR)+ perivascular stromal cells provide a major source of bone-forming osteoblasts in
商品
Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.
实验方案
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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