一般描述
肽-N-糖苷酶F（PNGase F）属于酶家族，主要用于N-连接聚糖的去糖基化。
应用
PNGase Fast可用于固定以进行去糖基化。它也可用于固定在基于甲基丙烯酸酯的整体载体上，以从糖蛋白释放N-连接的碳水化合物部分。
生化/生理作用
肽-N-糖苷酶F（PNGase F）从糖蛋白上裂解天冬酰胺连接的高甘露糖，杂合和复杂寡糖。它也可以使天冬酰胺脱氨基为天冬氨酸。PNGase Fast能够对抗体和免疫球蛋白融合蛋白，以及其他糖蛋白进行完整和快速的去糖基化，以准备用于下游层析或质谱分析。PNGase Fast创建了优化的工作流程，在不损害灵敏度或重现性的情况下减少了处理时间。
WGK
WGK 3
法规信息
常规特殊物品
David A Fischler et al.
Journal of biomolecular techniques : JBT, 30(4), 58-63 (2019-10-11)
There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization. One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F). A less expensive and quicker alternative has been reported
N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols
Fischler DA and Orlando R
Journal of biomolecular techniques : JBT, 30, 58-58 (2019)
Oriented immobilization of peptide-N-glycosidase F on a monolithic support for glycosylation analysis
Krenkova J, et al.
Journal of Chromatography A, 1322, 54-61 (2013)
Multidimensional system enabling deglycosylation of proteins using a capillary reactor with peptide-N-glycosidase F immobilized on a porous polymer monolith and hydrophilic interaction liquid chromatography-mass spectrometry of glycans
Krenkova J, et al.
Journal of Chromatography A, 1216, 3252-3259 (2009)
Jana Krenkova et al.
Journal of chromatography. A, 1322, 54-61 (2013-11-19)
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate moieties from glycoproteins. The glutathione-S-transferase-fusion PNGase F (PNGase F-GST) was
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