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Merck
CN

EMS0001

PNGase Fast

recombinant, expressed in E. coli

别名:

N-糖苷酶 F, 缩氨酸-N-糖苷酶F, 肽N-糖苷酶

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UNSPSC代码:
41131616

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重组

expressed in E. coli

质量水平

200

偶联物

(N-linked)

等级

Proteomics Grade

表单

ready-to-use solution

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

肽-N-糖苷酶F（PNGase F）属于酶家族，主要用于N-连接聚糖的去糖基化。

应用

PNGase Fast可用于固定以进行去糖基化。它也可用于固定在基于甲基丙烯酸酯的整体载体上，以从糖蛋白释放N-连接的碳水化合物部分。

生化/生理作用

肽-N-糖苷酶F（PNGase F）从糖蛋白上裂解天冬酰胺连接的高甘露糖，杂合和复杂寡糖。它也可以使天冬酰胺脱氨基为天冬氨酸。PNGase Fast能够对抗体和免疫球蛋白融合蛋白，以及其他糖蛋白进行完整和快速的去糖基化，以准备用于下游层析或质谱分析。PNGase Fast创建了优化的工作流程，在不损害灵敏度或重现性的情况下减少了处理时间。

储存分类代码

10 - Combustible liquids

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number
SLBV7894

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Multidimensional system enabling deglycosylation of proteins using a capillary reactor with peptide-N-glycosidase F immobilized on a porous polymer monolith and hydrophilic interaction liquid chromatography-mass spectrometry of glycans
Krenkova J, et al.
Journal of Chromatography A, 1216, 3252-3259 (2009)
Jana Krenkova et al.
Journal of chromatography. A, 1322, 54-61 (2013-11-19)
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate moieties from glycoproteins. The glutathione-S-transferase-fusion PNGase F (PNGase F-GST) was
David A Fischler et al.
Journal of biomolecular techniques : JBT, 30(4), 58-63 (2019-10-11)
There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization. One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F). A less expensive and quicker alternative has been reported
Jana Krenkova et al.
Journal of chromatography. A, 1216(15), 3252-3259 (2009-03-10)
A reactor with immobilized peptide-N-glycosidase F on a monolithic polymer support in a capillary has been developed that allows fast and efficient release of N-linked glycans from immunoglobulin G molecules. Two different monolithic scaffolds based on poly(glycidyl methacrylate-co-ethylene dimethacrylate) and
N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols
Fischler DA and Orlando R
Journal of biomolecular techniques : JBT, 30, 58-58 (2019)
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商品

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Correlation of FcR Affinity Chromatography with Glycan Pattern and ADCC Activity of a Therapeutic Antibody

Fast assessment of antibody-dependent cell-mediated cytotoxicity (ADCC) and glycoform pattern of a therapeutic antibody rituximab by FcγRIIIa affinity chromatography.

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