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Merck
CN
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安全信息

E8022

Sigma-Aldrich

pRc/RSV Expression vector

set of expression vectors powered by either the CMV2 or the RSV promoter

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MDL编号:
MFCD01094969
UNSPSC代码:
12352200
NACRES:
NA.85

等级

for molecular biology

形式

buffered aqueous solution

储存温度

−20°C

相关类别

一般描述

pRc and pRSV Supercoiled Expression Vectors (5.2 kb) are for protein expression in mammalian cells. Each vector contains:
  • The polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
  • The SV40 replication origin for episomal replication
  • The f1 origin for rescue of the sense strand
  • The neomycin resistance gene, and
  • An ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.


The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.

The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.

特异性

The MCS contains unique ApaI (CMV2 only), HindIII, NotI, SpeI (RSV only), and XbaI restriction sites, plus redundant BstXI sites.

特点和优势

Supercoiled vectors for protein expression in mammalian cells. Both of these vectors contain:
  • the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
  • the SV40 replication origin for episomal replication
  • the f1 origin for rescue of the sense strand
  • the neomycin resistance gene, and
  • an ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
The primary difference between these expression vectors is that one contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and the other from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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