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Merck
CN

E5134

Sigma-Aldrich

乙二胺四乙酸 二钠盐 二水合物

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

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别名:
Sequestrene Na₂, EDTA 二钠盐, EDTA-Na2, 乙二胺四乙酸Na2, 乙二胺四乙酸盐 二钠盐 二水合物, 二钠乙二胺四乙酸盐 二水合物, 依地酸钙钠
经验公式(希尔记法):
C10H14N2Na2O8 · 2H2O
CAS号:
分子量:
372.24
Beilstein:
3900609
EC 号:
UNSPSC代码:
12352107
PubChem化学物质编号:
NACRES:
NA.31

等级

for molecular biology

质量水平

检测方案

99.0-101.0% (titration)

形式

powder

反应适用性

reagent type: chelator

技术

electrophoresis: suitable

杂质

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

溶解性

3  M NaOH: 1.6 g/10 mL

痕量阳离子

Fe: ≤0.005%
Pb: ≤0.002%

适用性

suitable for electrophoresis
suitable for molecular biology

异质活性

DNase, RNase, NICKase and protease, none detected

储存温度

room temp

SMILES字符串

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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一般描述

脱水EDTA二钠盐可螯合金属离子,与二价阳离子形成八面体络合物。在分子生物学研究中可用于抑制依赖金属离子激活的酶。在诊断检测中,可作为采血抗凝剂用于血液学检验。

应用

EDTA二钠盐在研究中的应用:
  • 作为电泳、细胞培养、酶法检测、siRNA、流式细胞术、二甲基亚甲蓝等的缓冲液组分
  • 作为全血采集的抗凝剂
  • 作为细胞培养物样本冷冻的稳定剂
  • 与PBS和胰蛋白酶共同用于消化细胞
  • 用于污染土壤的重金属植物萃取
二价阳离子的螯合剂。 对需要二价阳离子发挥活性的酶,比如金属蛋白酶具有抑制作用。

特点和优势

  • 分子生物学级,适于许多分子生物学应用
  • 不含内切核酸酶DNA酶、RNA酶、切口酶和蛋白酶

其他说明

For additional information on our range of Biochemicals, please complete this form.

象形图

Exclamation markHealth hazard

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

靶器官

Respiratory Tract

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can solutions of Product E5134, EDTA disodium salt, be autoclaved?

    Yes.

  4. Is Product E5134, EDTA disodium salt, stable in solution?

    Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.

  5. What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?

    The optimal concentration is 1.5 mg per mL of blood.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Fine P et al.
The Science of the Total Environment, 493, 995-1005 (2014)
External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Ana Amaral et al.
Animals : an open access journal from MDPI, 11(1) (2021-01-21)
Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives
External quality assessment of methylmalonic acid and total homocysteine
Clinical Chemistry, 45, 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.

实验方案

本协议描述了一种使用甲醛完成化学交联蛋白质的方法。除零长交联剂外,甲醛是交联跨度最短(~2-3 Å)的交联剂,因此使其成为非常可靠地检测特定蛋白质-蛋白质相互作用的理想工具。

Formaldehyde cross-linking protocol detects specific protein interactions with confidence.

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

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