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用途
sufficient for 50 purifications
质量水平
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
环保替代产品分类
, Aligned
一般描述
Norgen Biotek植物/真菌总RNA纯化试剂盒可以快速地从多种植物和丝状真菌种样本中分离和纯化出总RNA（包括病毒RNA）。通过该试剂盒，可从新鲜或冰冻的植物组织、植物细胞或丝状真菌样本之中纯化出总RNA。大到mRNA和rRNA，小到miRNA和siRNA，所有大小的RNA均可通过该试剂盒进行纯化。 该试剂盒不需要使用液氮来均质化样本，操作步骤快速、方便。
应用
用于从植物和丝状真菌中快速纯化总RNA（包括microRNA）。
纯化所得的RNA具有最高的质量，可用于诸多下游应用，包括：
- qRT-PCR
- 反转录PCR
- Northern杂交
- RNAse保护
- 引物延伸
- 表达阵列分析
- 二代测序
特点和优势
- 可从广泛的植物和丝状真菌样本中分离RNA。
- 配套的稳定裂解缓冲液适于处理松针或葡萄叶片等高难度样本。
- 可从大分子量mRNA、microRNA和病毒RNA之中分离出多种RNA。
- 可纯化出高得率、优质的RNA。
- 无需进行苯酚/氯仿萃取。
- 无需采用液态进行均质化
其他说明
存储条件和产品稳定性
所有溶液应严格密封并在室温下储存。在未开封条件下，此类试剂至少可稳定保存1年。
所有溶液应严格密封并在室温下储存。在未开封条件下，此类试剂至少可稳定保存1年。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。
警示用语：
Warning
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
I El-Sharkawy et al.
Journal of experimental botany, 60(3), 907-922 (2009-02-14)
Seven ERF cDNAs were cloned from two Japanese plum (Prunus salicina L.) cultivars, 'Early Golden' (EG) and 'Shiro' (SH). Based on the sequence characterization, these Ps-ERFs could be classified into three of the four known ERF families. Their predicted amino
Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms.
Takao Ito et al.
Archives of virology, 158(1), 273-275 (2012-08-29)
I El-Sharkawy et al.
Journal of experimental botany, 63(3), 1225-1239 (2011-11-15)
Several dwarf plum genotypes (Prunus salicina L.), due to deficiency of unknown gibberellin (GA) signalling, were identified. A cDNA encoding GA 2-oxidase (PslGA2ox), the major gibberellin catabolic enzyme in plants, was cloned and used to screen the GA-deficient hybrids. This
I El-Sharkawy et al.
Journal of experimental botany, 61(6), 1761-1770 (2010-03-06)
Germin-like proteins (GLPs) have several proposed roles in plant development and defence. Two novel genes (Ps-GLP1 and 2) encoding germin-like protein were isolated from plum (Prunus salicina). Their regulation was studied throughout fruit development and during ripening of early and
Hila Yaffe et al.
BMC research notes, 5, 45-45 (2012-01-21)
Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues
实验方案
The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
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