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Merck
CN

D9154

邻联茴香胺 二盐酸盐

tablet

别名:

3,3'-二甲氧基联苯胺 二盐酸盐, 固蓝 B

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关于此项目

经验公式（希尔记法）:
C14H16N2O2 · 2HCl
化学文摘社编号:
20325-40-0
分子量:
317.21
NACRES:
NA.83
PubChem Substance ID:
24894263
UNSPSC Code:
12352204
EC Number:
243-737-5
MDL number:
MFCD00012488
Beilstein/REAXYS Number:
3917996

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产品名称

邻联茴香胺 二盐酸盐, tablet, 10 mg substrate per tablet

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

form

tablet

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

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相关类别

Biochem/physiol Actions

邻联茴香胺（3,3′-dimethoxybenzidine）是一种适合在 ELISA 步骤中使用的过氧化物酶底物。该底物产生可溶性终产物，颜色为黄橙色，可在 405 nm 处用分光光度法读取。可用 5 MHCl 终止反应。

Disclaimer

可能致癌。

Preparation Note

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000390713
0000390713
SLCJ7840
SLCD6994
SLBX3894

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M Kaszuba et al.
Biochimica et biophysica acta, 1419(2), 221-228 (1999-07-17)
Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated
Frédéric Frottin et al.
Molecular & cellular proteomics : MCP, 5(12), 2336-2349 (2006-09-12)
Methionine aminopeptidase (MAP) is a ubiquitous, essential enzyme involved in protein N-terminal methionine excision. According to the generally accepted cleavage rules for MAP, this enzyme cleaves all proteins with small side chains on the residue in the second position (P1')
Rémy Ricoux et al.
Bioconjugate chemistry, 19(4), 899-910 (2008-03-08)
To develop artificial hemoproteins that could lead to new selective oxidation biocatalysts, a strategy based on the insertion of various iron-porphyrin cofactors into Xylanase A (Xln10A) was chosen. This protein has a globally positive charge and a wide enough active
Cathrine Nordgaard et al.
International journal of molecular sciences, 22(17) (2021-09-11)
p38 and c-Jun N-terninal kinase (JNK) are activated in response to acute stress and inflammatory signals. Through modification of a plethora of substrates, these kinases profoundly re-shape cellular physiology for the optimal response to a harmful environment and/or an inflammatory
Iain S MacPherson et al.
Protein engineering, design & selection : PEDS, 23(3), 137-145 (2010-01-20)
Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput
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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

实验方案

Enzymatic Assay of Cholesterol Oxidase

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

胆固醇氧化酶的酶学测定

本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。

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