D8764
脱氧核糖核酸酶II 来源于牛脾脏
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
别名:
DNase II, 脱氧核苷酸酶 3′-寡核苷酸脱氢酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
Specific activity:
≥1,000 units/mg protein
Biological source:
bovine spleen
Concentration:
≥50% protein
产品名称
脱氧核糖核酸酶II 来源于牛脾脏, Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
biological source
bovine spleen
type
Type V
form
essentially salt-free, lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
38 kDa
concentration
≥50% protein
technique(s)
DNA extraction: suitable
tissue culture: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
RNase ≤0.1%
storage temp.
−20°C
Quality Level
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Application
牛脾脱氧核糖核酸酶Ⅱ已用于：
- 作为从结肠样品分离免疫细胞的消化混合液组分
- 消化视网膜外植体以制备单细胞悬液
- 分离人结肠组织的基质细胞
- 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分
Biochem/physiol Actions
脱氧核糖核酸酶II，也称为酸性DNA酶，可水解天然和变性DNA中的脱氧核糖核苷酸连接键，产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。
Other Notes
在 pH 4.6 25℃条件下，一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA260；[Mg2+] = 0.83 mM
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
Therese Ostberg et al.
Arthritis and rheumatism, 62(10), 2963-2972 (2010-06-10)
High mobility group box chromosomal protein 1 (HMGB-1) is a DNA binding nuclear protein that can be released from dying cells and activated myeloid cells. Extracellularly, HMGB-1 promotes inflammation. Clinical and experimental studies demonstrate that HMGB-1 is a pathogenic factor
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