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安全信息

D8437

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, sterile liquid, suitable for cell culture

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别名:
DME/F-12, 1:1混合
MDL编号:
MFCD00217417
UNSPSC代码:
12352207
NACRES:
NA.75

product name

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

杜氏改良Eagle培养基（DMEM）是一种富含能量的常用细胞培养基，包含葡萄糖、维生素和氨基酸。杜氏改良Eagle培养基（DMEM）与Ham F-12的1:1营养混合物常用于细胞培养研究。为了弥补去除血清造成的缓冲能力损失，可以在配方中以15 mM的最终浓度添加4-(2-羟乙基)-1-哌嗪乙磺酸（HEPES）缓冲液。

应用

杜氏改良Eagle培养基/营养混合物F-12 Ham：
  • 已用作培养基的组分，以培养原代培养激活的人肝星状细胞
  • 已用于培养软骨切片
  • 可用于考德里氏体的繁殖

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).

  4. What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.

  5. Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?

    When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).

  6. What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?

    We recommend storing Product No. D8437 at 2-8°C.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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