product name
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L
运输
ambient
储存温度
2-8°C
正在寻找类似产品? 访问 产品对比指南
一般描述
在成分确定的培养基研究中，通常用成分确定的混合物（包括营养素、生长因子和激素）替代血清添加到培养基中。常用的组合是Dulbecco′s改良Eagle培养基(DME)与Ham′s F-12营养混合物（1:1混合）。为了补偿去血清后的缓冲能力损失，可在配方中添加15 mM终浓度的HEPES缓冲液，帮助研究人员优化缓冲体系。
应用
Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于：
- 维持人肺和结直肠癌细胞系生存，进行次级代谢产物的生物学评估
- 培养人肾-2细胞(HK-2)并作为检测培养基组分，用于溶血磷脂酰胆碱(LPC)处理
- 小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
A new macrolide isolated from the endophytic fungus Colletotrichum sp
Bungihan ME, et al.
3rd Kuala Lumpur International Conference on Biomedical Engineering 2006: Biomed 2006, 6(1), 57-73 (2013)
Hayato Fukusumi et al.
PeerJ, 6, e4187-e4187 (2018-01-10)
Since the development of human-induced pluripotent stem cells (hiPSCs), various types of hiPSC-derived cells have been established for regenerative medicine and drug development. Neural stem/progenitor cells (NSPCs) derived from hiPSCs (hiPSC-NSPCs) have shown benefits for regenerative therapy of the central
Tijmen H Booij et al.
SLAS discovery : advancing life sciences R & D, 22(8), 974-984 (2017-06-24)
Polycystic kidney disease (PKD) is a prevalent disorder characterized by renal cysts that lead to kidney failure. Various signaling pathways have been targeted to stop disease progression, but most interventions still focus on alleviating PKD-associated symptoms. The mechanistic complexity of
Mariama Jaiteh et al.
Journal of medicinal chemistry, 61(12), 5269-5278 (2018-05-25)
Modulation of multiple biological targets with a single drug can lead to synergistic therapeutic effects and has been demonstrated to be essential for efficient treatment of CNS disorders. However, rational design of compounds that interact with several targets is very
Maria Vomero et al.
Scientific reports, 8(1), 14749-14749 (2018-10-05)
Neural interfaces for neuroscientific research are nowadays mainly manufactured using standard microsystems engineering technologies which are incompatible with the integration of carbon as electrode material. In this work, we investigate a new method to fabricate graphitic carbon electrode arrays on
商品
Nutrient Mixture F-12 Ham Formulation
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门