质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes
运输
ambient
储存温度
2-8°C
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应用
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。
数量
按照每升培养基13.5克粉末配制。
重悬
补充 3.7g/L 碳酸氢钠
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法规信息
动植物来源培养基
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性,应注意防潮。
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