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Merck
CN

D7777

Sigma-Aldrich

杜氏改良 Eagle 培养基 - 高葡萄糖

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

形式

powder

技术

cell culture | mammalian: suitable

组分

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

运输

ambient

储存温度

2-8°C

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应用

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

数量

按照每升培养基13.5克粉末配制。

重悬

补充 3.7g/L 碳酸氢钠

WGK

WGK 1

法规信息

动植物来源培养基

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实验方案

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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