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质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes
运输
ambient
储存温度
2-8°C
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应用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
数量
按照每升培养基13.5克粉末配制。
重悬
补充 3.7g/L 碳酸氢钠
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储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
法规信息
动植物来源培养基
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Assaf Almog et al.
PLoS computational biology, 15(5), e1006934-e1006934 (2019-05-03)
Neural systems are organized in a modular way, serving multiple functionalities. This multiplicity requires that both positive (e.g. excitatory, phase-coherent) and negative (e.g. inhibitory, phase-opposing) interactions take place across brain modules. Unfortunately, most methods to detect modules from time series
Emilia Galli et al.
Cell transplantation, 28(4), 413-424 (2019-03-08)
Cerebral dopamine neurotrophic factor (CDNF) shows beneficial effects in rodent models of Parkinson's and Alzheimer's disease. The brain is a challenging target for protein therapy due to its exclusive blood-brain barrier. Hence, the therapeutic protein should be delivered directly to
Maria H Jäntti et al.
FEBS open bio, 8(5), 817-828 (2018-05-11)
Prostate cancer is one of the most common cancers in men. Although it has a relatively high 5-year survival rate, development of resistance to standard androgen-deprivation therapy is a significant clinical problem. Therefore, novel therapeutic strategies are urgently needed. The
Otávio Valério de Carvalho et al.
Virology journal, 14(1), 124-124 (2017-06-28)
Canine distemper (CD) is a widespread infectious disease that can severely impact a variety of species in the order Carnivora, as well as non-carnivore species such as non-human primates. Despite large-scale vaccination campaigns, several fatal outbreaks have been reported in
Jie Wang et al.
Molecular medicine reports, 22(2), 1111-1118 (2020-07-07)
Neuromyelitis optica (NMO) is a severe neurological demyelinating autoimmune disease that affects the optic nerves and spinal cord. There is currently no effective cure or therapy. Aquaporin‑4 (AQP4) is a known target of the autoimmune antibody NMO‑IgG. Therefore, binding of
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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