产品名称
JumpStart ™ AccuTaq ™ LA DNA 聚合酶, Hot-start high fidelity Taq enzyme, 10X buffer included
feature
hotstart
Long & Accurate PCR
dNTPs included: no
form
liquid
usage
sufficient for 125 reactions
sufficient for 500 reactions
concentration
2.5 unit/μL
technique(s)
PCR: suitable
color
colorless
input
purified DNA
suitability
suitable for PCR
shipped in
wet ice
storage temp.
−20°C
Quality Level
Features and Benefits
- JumpStart AccuTaq LA DNA 聚合酶是一种抗体失活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量和特异性
- 保真度比 Taq DNA 聚合酶高 6.5 倍，使其成为多重 PCR 的首选酶
- 使用基因组模板产生高达 22 kb 的扩增子，使用不太复杂的模板（如 λ 或细菌基因组 DNA）产生高达 40 kb 的扩增子
- 减少设置时间，并消除与手动或蜡热启动方法相关的问题
Application
- 放大土壤DNA样品的聚合酶链式反应(PCR)
- 组蛋白H2A.Z的精确长范围PCR，细菌人工染色体(BAC)
- 作为PCR反应混合物的成分，通过常规PCR扩增病毒DNA
General description
Legal Information
Other Notes
Packaging
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
10 - Combustible liquids
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
商品
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。
实验方案
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
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