grade
Molecular Biology
form
liquid
usage
250 uses (25 μg size), 50 uses (10 μg size)
suitability
suitable for electrophoresis (DNA)
shipped in
dry ice
storage temp.
−20°C
General description
Sigma′s DNA Ladder (Supercoiled) contains 11 supercoiled DNA fragments from 2,067 – 16,210 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Other Notes
Contains 11 supercoiled fragments, 2,067–16,210 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Sigma′s DNA Ladder (Supercoiled) is provided in a solution of 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Michael J Molloy et al.
Nucleic acids research, 32(16), e129-e129 (2004-09-11)
Within the biopharmaceutical industry, recombinant plasmid DNA is used both as a raw material (e.g. in lentiviral and AAV vector production) as well as an active ingredient (e.g. in DNA vaccines). Consequently, many analytical laboratories are routinely involved with plasmid
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