应用
2′-脱氧鸟苷5′-三磷酸钠盐水合物已用于:
- 用作聚合酶链式反应 (PCR) 混合物中的一种成分,使用AM-毒素引物形成 PCR 产物
- 用作内标,提高毛细管电泳 (CE) 分析的分析性能,评估纯化工艺
- 用作体外酶活性测定中的推定底物,与Nudix水解酶 15 (NUDT15) 蛋白一起孵育,评估推定的 NUDT15 抑制剂
生化/生理作用
脱氧鸟苷5′-三磷酸(dGTP),一种嘌呤脱氧核糖核苷酸,是DNA聚合酶和逆转录酶的底物。 dGTP可用于研究各种核苷三磷酸酶(NTPase)和核糖核苷酸还原酶的分布、特异性和动力学。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, type N95 (US)
PloS one, 6(7), e22336-e22336 (2011-08-04)
Chikungunya virus (CHIKV) is an insect borne virus (genus: Alphavirus) which causes acute febrile illness in humans followed by a prolonged arthralgic disease that affects the joints of the extremities. Re-emergence of the virus in the form of outbreaks in
Cell chemical biology, 28(12), 1693-1702 (2021-07-01)
Ganciclovir (GCV) is the first-line therapy against human cytomegalovirus (HCMV), a widespread infection that is particularly dangerous for immunodeficient individuals. Closely resembling deoxyguanosine triphosphate, the tri-phosphorylated metabolite of GCV (GCV-TP) is preferentially incorporated by the viral DNA polymerase, thereby terminating
The Journal of biological chemistry, 286(36), 31490-31500 (2011-07-23)
During DNA synthesis, DNA polymerases must select against ribonucleotides, present at much higher levels compared with deoxyribonucleotides. Most DNA polymerases are equipped to exclude ribonucleotides from their active site through a bulky side chain residue that can sterically block the
Analytical biochemistry, 335(1), 150-161 (2004-11-03)
A novel approach for quantitation of DNA (oligonucleotides) with an unprecedented accuracy of approximately 1% is reported. Quantitation of DNA is commonly performed by measuring UV absorption or fluorescence from dyes intercalated into DNA. Both methods need accurate quantitation standards
Proceedings of the National Academy of Sciences of the United States of America, 108(24), 9815-9820 (2011-06-02)
Human ribonucleotide reductases (hRNRs) catalyze the conversion of nucleotides to deoxynucleotides and are composed of α- and β-subunits that form active α(n)β(m) (n, m = 2 or 6) complexes. α binds NDP substrates (CDP, UDP, ADP, and GDP, C site)
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