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应用
适用于：
- 平端和粘性DNA片段的连接
- 克隆载体和限制性插入片段的连接
- 双链DNA和RNA或DNA/RNA杂合体中的缺口补平
- 通过桥连寡核苷酸接头来偶联RNA单链
生化/生理作用
T4 DNA连接酶可在双链体DNA的相邻多核苷酸的末端之间形成能量依赖性的磷酸二酯键。连接反应需要ATP作为辅助因子。平末端片段的连接需要更高的酶浓度并可通过在反应混合物中使用PEG来促进。该酶需要3′羟基和5′磷酸基用于连接。可通过用碱性磷酸酶进行去磷酸化来防止载体DNA的自连。T4连接酶在DNA和RNA缺口的修复中可发挥积极作用。
组分
T4 DNA连接酶以溶于20 mM Tris-HCl（pH 7.5），50 mM KCl，1 mM DTT和50%（v/v）甘油的溶液形式提供。
单位定义
一个Weiss单位的定义是在37℃ 下，在20分钟内催化1 nmol的P32从焦磷酸盐交换至ATP作为Norit可吸收物质所需的酶量。
其他说明
T4 DNA连接酶可通过在65 °C加热10分钟灭活。
相关产品
产品编号
说明
价格
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
Justin L Sparks et al.
Molecular cell, 47(6), 980-986 (2012-08-07)
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Athena Kantartzis et al.
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Adam B Robertson et al.
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Ayako Nishizawa-Yokoi et al.
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