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D2438

Sigma-Aldrich

二甲基亚砜

sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

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别名:
DMSO, 甲基亚砜
线性分子式:
(CH3)2SO
CAS号:
67-68-5
分子量:
78.13
Beilstein:
506008
EC 号:
200-664-3
MDL编号:
MFCD00002089
UNSPSC代码:
12191502
PubChem化学物质编号:
24893688
NACRES:
NA.75

等级

BioPerformance Certified

质量水平

400

Agency

USP XXIV
meets EP testing specifications
meets USP testing specifications

蒸汽密度

2.7 (vs air)

蒸汽压

0.42 mmHg ( 20 °C)

无菌性

sterile-filtered

形式

liquid

自燃温度

573 °F

expl. lim.

42 %, 63 °F

技术

cell culture | hybridoma: suitable
cryopreservation: suitable

杂质

≤0.1% water

颜色

colorless

折射率

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

溶解性

H2O: miscible (completely)

密度

1.10 g/mL (lit.)

SMILES字符串

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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相关类别

一般描述

二甲基亚砜（DMSO）是一种非质子溶剂。不溶于水的治疗性和有毒分子通常溶于DMSO。液氮与诸如DMSO的冷冻保护剂结合使用是广泛使用的细胞保存方法。DMSO已用于制备多种分子的储备溶液，并同时也作为这些分子的对照。它已用于溶解迁移后细胞的细胞迁移试验。经BPC测试的DMSO也可满足生物技术和组织工程应用的要求。

应用

二甲基亚砜已被用在体内培养和诱导肝星状细胞（HSC）活化中的载体对照。它还被用于制备储备溶液，以研究阿魏酸（FA）对髓核（NP）细胞的细胞毒性。

特点和优势

即用型无菌过滤产品，被方便地包装在隔垫螺帽琥珀色瓶中。 符合USP和EP检测的要求

注意

在室温下容易过冷并缓慢重融。 固化产品可以通过加热至室温来重新液化，而不会损害产品。

WGK

WGK 1

闪点(°F)

188.6 °F

闪点(°C)

87 °C

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  4. What concentration of DMSO do I use in a cell freezing media?

    DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?

    Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.

  7. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  8. What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?

    The industry standard for 'endotoxin free' is less than 1 EU/mL.

  9. What is the difference between Dimethyl sulfoxide (DMSO) Products. D1435 and D2438?

    Products D1435 and D2438 both meet the USP specifications. The D2438 is sterile-filtered, but D1435 is not. Furthermore, the D2438 is quality control tested for endotoxin and use-tested for cell freezing.

  10. What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

  11. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  12. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  13. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Björn Zörner et al.
Brain : a journal of neurology, 137(Pt 6), 1716-1732 (2014-04-17)
Anatomical plasticity such as fibre growth and the formation of new connections in the cortex and spinal cord is one known mechanism mediating functional recovery after damage to the central nervous system. Little is known about anatomical plasticity in the
Mulmudi Hemant Kumar et al.
Advanced materials (Deerfield Beach, Fla.), 26(41), 7122-7127 (2014-09-13)
Lead free perovskite solar cells based on a CsSnI3 light absorber with a spectral response from 950 nm is demonstrated. The high photocurrents noted in the system are a consequence of SnF2 addition which reduces defect concentrations and hence the
C F Brayton
The Cornell veterinarian, 76(1), 61-90 (1986-01-01)
Dimethyl sulfoxide (DMSO) is a very simple compound that has stimulated much controversy in the scientific and popular literature. Fig. 1 It is an aprotic solvent. Therapeutic and toxic agents that are not soluble in water are often soluble in
Phillip McDonagh et al.
Veterinary microbiology, 174(3-4), 438-447 (2014-12-04)
Feline infectious peritonitis (FIP), a feline coronavirus (FCoV) induced disease, is almost invariably fatal with median life expectancy measured in days. Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen
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