所有图片(1)
别名:
4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM
经验公式（希尔记法）:
C21H14F2N2O5
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质量水平
检测方案
≥97% (HPLC)
形式
DMSO solution
储存条件
desiccated
颜色
colorless to yellow, including pink
运输
wet ice
储存温度
−20°C
InChI
1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
InChI key
DIJCILWNOLHJCG-UHFFFAOYSA-N
应用
DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.
生化/生理作用
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
特点和优势
This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Detection of protein S-nitrosothiols (SNOs) in plant samples on diaminofluorescein (DAF) gels
Rodriguez-Ruiz M, et al.
Bio-protocol null
Marta Rodríguez-Ruiz et al.
Bio-protocol, 7(18), e2559-e2559 (2017-09-20)
In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (
商品
We offer many products related to nitric oxide synthases for your research needs.
我们针对客户的研究需求，提供了许多一氧化氮合酶相关的产品。
一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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