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Merck
CN

D1946

Sigma-Aldrich

二氨基荧光素-FM 二乙酸酯

≥98% (HPLC)

别名:

4-氨基-5-(N-甲氨基)-3′,6'-双(乙酰氧基)-2′,7'-二氟-螺[ 异苯并呋喃-1 (3H) ,9'-[9H]黄原酮]-3-酮, DAF-FM DA

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About This Item

经验公式(希尔记法):
C25H18F2N2O7
分子量:
496.42
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.77

质量水平

方案

≥98% (HPLC)

表单

film

储存条件

desiccated

颜色

colorless to yellow

荧光

λex 500 nm; λem 515 nm in DMSO

运输

wet ice

储存温度

−20°C

SMILES字符串

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key

BEVHTVRRVVEMEF-UHFFFAOYSA-N

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应用

DAF-FM DA 已被用于测量肝组织匀浆中的一氧化氮 (NO) 水平。它还被用于检测人脐静脉内皮细胞 (HUVEC) 的 NO 染色。

生化/生理作用

DAF-FM DA 是一种用于检测一氧化氮 (NO) 的细胞渗透性荧光探针。DAF-2 是最初的化合物,应用广泛,但 DAF-FM 和 DAF-FM DA 与 DAF-2 相比对一氧化氮 (NO) 更敏感,更耐光且pH 敏感性较低。DAF-FM DA 能很好地渗透到活细胞中,并通过胞浆酯酶迅速转化为水溶性 DAF-FM。

特点和优势

该化合物在受体分类和信号转导手册的一氧化氮合酶页面上有重点介绍。想要浏览手册的其他页面, 请单击此处

注意

吸湿

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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分析证书(COA)

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Verena K Capellini et al.
PloS one, 8(5), e62887-e62887 (2013-05-22)
It has been known for more than a century that pH changes can alter vascular tone. However, there is no consensus about the effects of pH changes on vascular response. In this study, we investigated the effects of extracellular pH
Shaoting Du et al.
Journal of experimental botany, 67(3), 893-904 (2015-11-27)
CO2 elevation often alters the plant's nitrate reductase (NR) activity, the first enzyme acting in the nitrate assimilation pathway. However, the mechanism underlying this process remains unknown. The association between elevated CO2-induced alterations of NR activity and nitric oxide (NO)
Christopher Groth et al.
Cancers, 13(4) (2021-02-14)
Despite recent improvement in the treatment of malignant melanoma by immune-checkpoint inhibitors, the disease can progress due to an immunosuppressive tumor microenvironment (TME) mainly represented by myeloid-derived suppressor cells (MDSC). However, the relative contribution of the polymorphonuclear (PMN) and monocytic
Good hydration and cell-biological performances of superparamagnetic calcium phosphate cement with concentration-dependent osteogenesis and angiogenesis induced by ferric iron
Zhang J, et al
Journal of Material Chemistry B: Materials for Biology and Medicine, 3(45), 8782-8795 (2015)
Bing Fang Luo et al.
Journal of experimental botany, 63(8), 3127-3136 (2012-03-02)
Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered

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