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Merck
CN

D1821

Sigma-Aldrich

DAF-FM

≥98% (HPLC)

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别名:
4-氨基-5-甲基氨基-2′,7′-二荧光素, 二氨基荧光素-FM
经验公式(希尔记法):
C21H14F2N2O5
分子量:
412.34
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.77

质量水平

检测方案

≥98% (HPLC)

形式

powder

储存条件

desiccated

颜色

white to brown

溶解性

DMSO: >1 mg/mL

运输

wet ice

储存温度

−20°C

SMILES字符串

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI key

DIJCILWNOLHJCG-UHFFFAOYSA-N

应用

DAF-FM已被用于小麦苗根部截面中一氧化氮(NO)的成像。它还被用于标记神经嵴(NC)细胞。

生化/生理作用

DAF-FM是一种高灵敏度、光稳定的荧光探针,可用于检测一氧化氮(NO)。DAF-2为初始化合物并且被广泛使用,但DAF-FM与DAF-2相比,对一氧化氮(NO)更敏感。DAF-FM比DAF-2具有更强的光稳定性以及更低的pH敏感性。DAF-FM不像二乙酸酯衍生物那样具有细胞渗透性,但它不需要被细胞溶质酶激活。

注意

吸湿的

制备说明

产品从白色到棕色的颜色变化不会改变其性能。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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