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质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high
运输
ambient
储存温度
2-8°C
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应用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
数量
按照每升培养基17.3克粉末配制。
重悬
补充3.7 g/L碳酸氢钠。
补充剂
产品编号
说明
价格
通常也和此产品一起购买
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Kiran Pandey et al.
Animal reproduction science, 181, 93-102 (2017-04-24)
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers
Farlen J B Miranda et al.
Poultry science, 95(1), 12-18 (2016-01-23)
Raising ostriches became an important economic activity after their products became commodities. The health of farm animals is of paramount importance, so assessing basic immunological responses is necessary to better understand health problems. We developed a method to obtain ostrich
Jiping Sun et al.
Journal of cellular and molecular medicine, 22(10), 4840-4855 (2018-07-20)
BMSCs are important in replacement therapy of diabetic nephropathy (DN). MiR-124a exerts effect on the differentiation capability of pancreatic progenitor cells. The objective of this study was to explore the molecular mechanisms, the functions of miR-124a and bone marrow mesenchymal
Importance of ROS-mediated autophagy in determining apoptotic cell death induced by physapubescin B.
Jian Xu et al.
Redox biology, 12, 198-207 (2017-03-05)
Physapubescin B, a steroidal compound extracted from the plant Physalis pubescens L. (Solanaceae), has been reported to possess anti-cancer potential, whereas the molecular mechanism remains elusive. In this study, we first demonstrated that physapubescin B induced autophagy in human cancer
Peiyi Yan et al.
The American journal of Chinese medicine, 46(3), 673-687 (2018-04-05)
Curcumin is a hydrophobic polyphenol derived from turmeric: the rhizome of the herb Curcumalonga. Autophagy is an evolutionarily conserved process, in which cellular proteins and organelles are engulfed in autophagosome and then fuses with lysosome for degradation. Our previous study
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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