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Merck
CN
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文件

CS0030

Sigma-Aldrich

衰老细胞组织化学染色试剂盒

sufficient for 100 tests

别名:

Senescent cells IHC kit, cellular β-galactosidase probe

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.32

用途

sufficient for 100 tests

质量水平

200

包装

pkg of 1 kit

储存条件

dry at room temperature

检测方法

colorimetric

运输

dry ice

储存温度

−20°C

相关类别

一般描述

衰老细胞组织化学染色试剂盒含有鉴定衰老细胞所需的所有试剂，测试基于pH 6下ß-半乳糖苷酶活性的组织化学染色。ß-衰老细胞中的半乳糖苷酶活性是可检测的，靜止細胞、永生细胞或肿瘤细胞则无法检测。
衰老细胞组织化学染色试剂盒含有鉴定衰老细胞所需的所有试剂，测试基于pH 6下β-半乳糖苷酶活性的组织化学染色。β-衰老细胞中的半乳糖苷酶活性是可检测的，靜止細胞、永生细胞或肿瘤细胞则无法检测。细胞衰老是一系列事件的进展，细胞借此由活跃分裂转为不分裂期。细胞衰老过程和老化相关。细胞分裂的减少几乎是不可逆的、完全的。除了丧失分裂能力外，细胞的形态学、形状和物理外观，以及它们的基因表达模式也发生变化。在该过程的结束时，细胞常常死亡，尽管细胞可能仍长期保持活力。

应用

衰老细胞组织化学染色试剂盒已被用于：
  • 与衰老相关的β-半乳糖苷酶测试
  • 测试人胰腺癌细胞
  • 脂肪源性干细胞（ADSC）
  • 脐血间充质/干细胞（CBMSC）中的细胞衰老

生化/生理作用

复制性衰老是一种生长停滞状态，主要表现为细胞失去分裂潜能，同时细胞形态、形状、生理特征和基因表达谱发生变化。

法律信息

本产品根据美国专利号：第5491069和5795728号授权生产。

仅试剂盒组分

产品编号
说明
  • X-gal solution 4 mL
  • Staining Solution, 10X 15 mL
  • Fixation Buffer 10x 15 mL
  • Reagent B 1.5 mL
  • Reagent C 1.5 mL
  • Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

相关产品

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

补充剂危害

EUH032

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product CS0030, Senescence Cells Histochemical Staining Kit be used with fixed cells/tissues?

    Product CS0030, Senescence Cells Histochemical Staining Kit was designed for use with fresh (not-fixed) cells.  The kit contains a fixation buffer to fix the cells.  It is 10% formaldehyde, 1% glutaraldehyde in DPBS at 1X.  The cells will be fixed prior to staining.  The staining will show the beta-galactosidase activity that was present at the time of fixation.We tested the kit only on cells and we have no experience with tissue sections. The following papers use an assay for ßGAL on tissue sections which suggests that the kit will work with tissue sections.Castro P, et al., Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia. Prostate, 60(2), 153-159 (2004).Chen, Z., et al., Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature, 436, 725-730 (2005).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
Mafalda Ramos de Matos et al.
Cancers, 11(3) (2019-03-23)
Intratumor genetic heterogeneity (ITH) is the main obstacle to effective cancer treatment and a major mechanism of drug resistance. It results from the continuous evolution of different clones of a tumor over time. However, the molecular features underlying the emergence
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