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Merck
CN

CS0013

Sigma-Aldrich

Red Protease Detection Kit

Fluorescent protease assay, high sensitivity

别名:

Fluorescent Protease Assay Kit, Protease Assay Kit

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About This Item

UNSPSC代码:
12350000
NACRES:
NA.51

质量水平

包装

pkg of 1 kit

运输

dry ice

储存温度

−20°C

一般描述

The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.

Fluorescence excitation wavelength = 535 nm

Fluorescence emission wavelength = 595 nm

应用

Detection and quantitation of protease activity in samples. Useful for impurity testing.

特点和优势

  • Fluorescence is pH-independent
  • No separation or precipation steps are required
  • High sensitivity and signal-to-noise

组分

CS0013A Trypsin (1 mg/mL): 100 μL

CS0013B Protease AssayBuffer × 2: 30 mL

CS0013C TAMRA-Casein substrate: 280 μL

适用性

Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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商品

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

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