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CS0005

Sigma-Aldrich

Cholesterol Quantification Assay kit

别名:

Cholesterol Assay kit, cholesteryl ester, free cholesterol, total cholesterol

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1 KIT
CN¥11,569.45
5 X 1 KIT
CN¥46,289.42

CN¥11,569.45

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1 KIT
CN¥11,569.45
5 X 1 KIT
CN¥46,289.42

About This Item

UNSPSC代码:
12352202
NACRES:
NA.83

CN¥11,569.45

请联系客服了解存货情况
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feature

CE compliant, powder-free: no, standard type EN 374 (cat. III), latex free, standard type EN 388 (cat. III), reusable

feature

CE compliant, latex free, powder-free: no, reusable, standard type EN 374 (cat. III), standard type EN 388 (cat. III)

feature

CE compliant, latex free, powder-free: no, reusable, standard type EN 374 (cat. III), standard type EN 388 (cat. III)

feature

CE compliant, latex free, powder-free: no, reusable, standard type EN 374 (cat. III), standard type EN 388 (cat. III)

material

black (fluorocarbon rubber), black gloves

material

black (fluorocarbon rubber), black gloves

material

black (fluorocarbon rubber), black gloves

material

black butyl rubber , black gloves

manufacturer/tradename

KCL 890

manufacturer/tradename

KCL 890

manufacturer/tradename

KCL 890

manufacturer/tradename

KCL 897

packaging

pack of 1 pair

packaging

pack of 1 pair

packaging

pack of 1 pair

packaging

pack of 1 pair

size

XL (11)

size

S (8)

size

L (10)

size

M

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

一般描述

Cholesterol is a lipid that constitutes 20-25% of the structural components of cell membranes. Cholesterol presence assure membrane structural fluidity. Cholesterol also plays a role as an intermediate in several hormones and bile acid biosynthesis and vitamin D. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane. The major sites of cholesterol synthesis are the small intestine and liver. Cholesterol circulates in the blood as both the free acid and as cholesterol esters. Increased cholesterol levels are associated with the development of atherosclerosis and cardiovascular diseases. Thus, serum cholesterol regulation has an important therapeutic role.

The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol, cholesteryl esters, or total cholesterol (which constitutes both free cholesterol and cholesteryl esters). The cholesterol concentration is determined via a coupled enzymatic reaction. This kit allows for either fluorometric or colorimetric detection, to allow maximum flexibility and a large detection range. The linear ranges are as follows:
  • Fluorescence assay: 0.1–0.5 μg
  • Colorimetric assay: 1–5 μg

应用

  • Measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples (For example: Serum and Plasma and tissue culture).
  • Measurement can be done either by fluorometric or colorimetric detection.
Suitable for the detection of free cholesterol, cholesteryl esters, or total cholesterol in biological samples.

特点和优势

  • Highly sensitive, simple and high-throughput assay for measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples
  • User friendly: No need to weigh or calculate needed amounts of reagents on your own
  • Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • Suitable for both colorimetric and fluorometric detection
  • More experiments in one kit - contains sufficient reagents for 100 colorimetric or 400 fluorometric tests.
  • Detection time: only 30 minutes

危险声明

H412

预防措施声明

P273 - P501

危险分类

Aquatic Chronic 3

储存分类代码

10 - Combustible liquids

法规信息

常规特殊物品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000461009
    0000461009
    0000412409
    0000412409
    0000352355

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    Min-Sik Kim et al.
    Journal of the American Society for Mass Spectrometry, 21(9), 1606-1611 (2010-05-21)
    Shotgun proteomics has been used extensively for characterization of a number of proteomes. High-resolution Fourier transform mass spectrometry (FTMS) has emerged as a powerful tool owing to its high mass accuracy and resolving power. One of its major limitations, however
    Mark V Ivanov et al.
    Journal of proteome research, 13(4), 1911-1920 (2014-02-28)
    Data-dependent tandem mass spectrometry (MS/MS) is one of the main techniques for protein identification in shotgun proteomics. In a typical LC-MS/MS workflow, peptide product ion mass spectra (MS/MS spectra) are compared with those derived theoretically from a protein sequence database.
    Henrik Molina et al.
    Analytical chemistry, 80(13), 4825-4835 (2008-06-11)
    Electron transfer dissociation (ETD) is a recently introduced mass spectrometric technique which has proven to be an excellent tool for the elucidation of labile post-translational modifications such as phosphorylation and O-GlcNAcylation of serine and threonine residues. However, unlike collision induced
    Leslie Muller et al.
    Proteomics, 16(23), 2953-2961 (2016-10-18)
    Sample preparation, typically by in-solution or in-gel approaches, has a strong influence on the accuracy and robustness of quantitative proteomics workflows. The major benefit of in-gel procedures is their compatibility with detergents (such as SDS) for protein solubilization. However, SDS-PAGE
    Markus Brosch et al.
    Molecular & cellular proteomics : MCP, 7(5), 962-970 (2008-01-25)
    It is a major challenge to develop effective sequence database search algorithms to translate molecular weight and fragment mass information obtained from tandem mass spectrometry into high quality peptide and protein assignments. We investigated the peptide identification performance of Mascot
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