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一般描述
叶绿体分离试剂盒为从植物叶片中分离完整的叶绿体提供了有效的方法。该方法包括机械式细胞壁和细胞膜破坏、过滤法细胞碎片收集和叶片组织、离心法叶绿体分离、采用Percoll®分层液或梯度法法从破碎的叶绿体之中分离完整的叶绿体。
应用
在叶绿体细胞器研究中，叶绿体分离试剂盒可用于从植物叶片中分离叶绿体以便进行结构和功能研究。
特点和优势
- 专门配制的缓冲液 - 采用易于执行的简单、成熟实验方案，节省时间
- 可分离完整的叶绿体 - 适于研究碳同化、电子流和磷酸化、代谢转运或蛋白质靶向等过程
- 总细胞器蛋白质 - 适于进行功能性研究、代谢转运、蛋白质组谱分析和基因组应用
- 规格方便 - 节省时间，减少浪费
适用性
叶绿体分离试剂盒已采用菠菜、豌豆、生菜、卷心菜、莙达菜和烟草进行过测试。
储存分类代码
10 - Combustible liquids
法规信息
新产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Paul E Abraham et al.
Nature plants, 2, 16178-16178 (2016-11-22)
Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3 and C4 photosynthesis) day/night pattern of stomatal closure/opening to shift CO2
Bruna Marques Dos Santos et al.
Journal of proteomics, 150, 252-257 (2016-10-25)
Photosynthetic organisms may be drastically affected by the future climate projections of a considerable increase in CO2 concentrations. Growth under a high concentration of CO2 could stimulate carbon assimilation-especially in C3-type plants. We used a proteomics approach to test the
Diana V Dugas et al.
Scientific reports, 5, 16958-16958 (2015-11-26)
The Leguminosae has emerged as a model for studying angiosperm plastome evolution because of its striking diversity of structural rearrangements and sequence variation. However, most of what is known about legume plastomes comes from few genera representing a subset of
Xiaoli Luo et al.
PloS one, 8(1), e54002-e54002 (2013-01-22)
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous
Sarah Conte et al.
Plant physiology, 151(2), 559-573 (2009-08-14)
Widespread antibiotic resistance is a major public health concern, and plants represent an emerging antibiotic exposure route. Recent studies indicate that crop plants fertilized with antibiotic-laden animal manure accumulate antibiotics; however, the molecular mechanisms of antibiotic entry and subcellular partitioning
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