质量水平
相关类别
一般描述
Imprint Ultra Chromatin IP 试剂盒是 Sigma′第二代染色质免疫沉淀（ChIP） 试剂盒，旨在实现最高灵敏度和最佳下一代测序结果。它提供了一套完整的染色质免疫沉淀解决方案，包括用于 DNA 纯化的色谱柱和试剂。该试剂盒让研究人员可以探索低丰度转录因子（TF） 的全基因组结合位点，以及新的组蛋白修饰。它针对来自 106 个细胞（最多约 50 μg DNA）的染色质的 ChIP 反应进行了优化，也可以扩大规模（或合并多个制剂）以适用于108 个细胞，用于 ChIP-chip 和 ChIP-Seq 应用中的全基因组结合研究。
应用
- 适用于下游应用
- 从单个靶标表征到全基因组分析的技术
- 信号转导途径的表征
- ChIp-chIP和ChIP-seq数据的验证
特点和优势
- 成功采用与低丰度、中等和高表达转录因子以及组蛋白修饰相关的 CHiP′ed DNA。
- 更大的容量 - 可用于 2-10 × 106 的各种细胞数目范围
- 最高的灵敏度 - 能够检测低至 2 × 106个细胞的低丰度转录因子
- 采用 DNA 封闭的 "Staph-Seq" 进行IP（免疫沉淀），最大限度减少下游 ChIP-Seq 应用中的葡萄球菌A DNA 污染。
法律信息
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
仅试剂盒组分
产品编号
说明
- Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
- Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- S6576ChIP Next Gen Seq Sepharose™化学品安全说明书
- I8896IGEPAL® CA-630, for molecular biology化学品安全说明书
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture化学品安全说明书
- W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder化学品安全说明书
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder化学品安全说明书
- A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)化学品安全说明书
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)化学品安全说明书
- D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization化学品安全说明书
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution化学品安全说明书
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution化学品安全说明书
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)化学品安全说明书
查看所有结果 (12)
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
靶器官
Central nervous system
储存分类代码
5.1A - Strongly oxidizing hazardous materials
法规信息
监管及禁止进口产品
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
实验方案
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
相关内容
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
蛋白质是细胞内部的主要功能分子，负责细胞功能和生存的多种生物学活动。值得关注的是，许多蛋白质也能与DNA发生相互作用。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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