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安全信息

CAS9RFPVP

Sigma-Aldrich

Cas9-RFP Lenti Plasmid

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选择尺寸

25 μL
¥2,257.75
100 μL
¥4,582.18
200 μL
¥5,503.01

¥2,257.75

国内现货，预计发货时间2025年4月27日详情

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25 μL
¥2,257.75
100 μL
¥4,582.18
200 μL
¥5,503.01

About This Item

UNSPSC代码:
41106609
NACRES:
NA.51

¥2,257.75

国内现货，预计发货时间2025年4月27日详情

获取大包装报价

重组

expressed in E. coli

表单

liquid

包装

vial of 50 μL

浓度

20 ng/μL in TE buffer; DNA (1μg of Plasmid DNA)

应用

CRISPR

报告基因

RFP

筛选方法

ampicillin

运输

dry ice

储存温度

−20°C

相关类别

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此商品
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Anti-Phosphoserine antibody, Mouse Monoclonal clone PSR-45, purified from hybridoma cell culture

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Anti-Phosphoserine antibody, Mouse Monoclonal

Anti-Phosphothreonine antibody, Mouse monoclonal clone PTR-8, purified from hybridoma cell culture

P6623

Anti-Phosphothreonine antibody, Mouse monoclonal

Anti-Phosphotyrosine antibody, Mouse monoclonal clone pT-154, purified from hybridoma cell culture

P1869

Anti-Phosphotyrosine antibody, Mouse monoclonal

Anti-Phosphotyrosine antibody, Mouse monoclonal clone PT-66, purified from hybridoma cell culture

P5872

Anti-Phosphotyrosine antibody, Mouse monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

-

biological source

mouse

clone

PSR-45, monoclonal

clone

PTR-8, monoclonal

clone

pT-154, monoclonal

clone

PT-66, monoclonal

technique(s)

dot blot: suitable, microarray: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, western blot: 2.5-5.0 μg/mL using total rat brain extract.

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1 μg/mL, microarray: suitable, western blot: 5-10 μg/mL using A431 cell extracts

technique(s)

direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

一般描述

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and RFP linked by a 2A peptide (EF1a-Cas9-2A-RFP) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-RFP lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed when packaged into lentiviral particles.

To order gRNA in any format click here

应用

Functional Genomics/Target Validation
  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Manufacture of Cas9-RFP expressing Lentiviral Particles

特点和优势

  • Highly specific
  • Highly active
  • Ready to use purified plasmid DNA

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

法律信息

CRISPR Use License Agreement

Lentiviral, WPRE and Evrogen Fluorophore Licenses

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    11192010MN

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