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表单
liquid
包装
pkg of 8 x 25 μL (aliquots)
浓度
≥5 x 10 8 VP/ml virus (via p24 assay)
应用
CRISPR
报告基因
GFP
运输
dry ice
储存温度
−70°C
1 of 4
此商品 | 27267U | 27185U | 27088U |
|---|---|---|---|
| volume 4 mL | volume 2 mL | volume 40 mL | volume 15 mL |
| feature closure type screw top vial | feature closure type screw top vial | feature closure type screw top vial | feature closure type screw top vial |
| fitting thread for 13-425 | fitting thread for 10-425 | fitting thread for 24-400 | fitting thread for 18-400 |
| material amber glass vial | material amber glass vial | material amber glass vial | material amber glass vial |
| packaging pkg of 100 ea | packaging pkg of 100 ea | packaging pkg of 100 ea | packaging pkg of 100 ea |
| O.D. × H × I.D. 15 mm × 45 mm × 8 mm | O.D. × H × I.D. 12 mm × 32 mm × 6 mm | O.D. × H × I.D. 29 mm × 82 mm × 17 mm | O.D. × H × I.D. 21 mm × 70 mm × 12 mm |
一般描述
Ready-to-use Cas9-GFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-GFP lentiviral particles efficiently and stably integrate Cas9 and GFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed.
To order gRNA in any format click here
To order gRNA in any format click here
应用
Functional Genomics/Target Validation
- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of cell lines stably expressing Cas9-GFP
特点和优势
- Highly specific
- Highly active
- Ready to infect/transduce
原理
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
单位定义
VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--病毒，疫苗等
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
相关内容
High-throughput reagents for drug target screening and immunogenicity testing.
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