重组
expressed in E. coli
质量水平
形式
lyophilized powder
比活
≥50 units/mg protein
分子量
64 kDa
组成
Protein, ≥15% biuret
储存温度
−20°C
应用
胆固醇氧化酶已用于研究中以证明疏水 - 亲水嵌段共聚物系统中的微观异质性可用于固定酶和进行酶促反应。胆固醇氧化酶也被用于一项研究，该研究得出结论，激活胆固醇的两亲可能有助于治疗鼻咽癌。
胆固醇氧化酶用于测定血清胆固醇。该酶还可应用于食品样品中类固醇的微量分析以及区分 3-酮类固醇和 3β-羟基类固醇。表达胆固醇氧化酶的转基因植物正在与棉铃象甲作斗争中进行研究。CHOD 也被用作分子探针来阐明细胞膜结构。
生化/生理作用
胆固醇氧化酶 (CHOD) 是一种含有 FAD 的单体黄素蛋白，其催化胆固醇分解代谢的第一步。这种双功能酶在需要 FAD 的步骤中将胆固醇氧化为胆甾-5-烯-3-酮，然后随着H2O2的释放而异构化为胆甾-4-烯-3-酮。
物理属性
含 FAD 辅因子的 Ⅱ 型胆固醇氧化酶与该酶共价连接。
单位定义
在 pH 值为 7.5、25°C 条件下，每分钟 1 个单位的胆固醇将转化为 4-胆甾烯-3-酮 (1.0 μmol/min)。注：4-胆甾烯-3-酮可能发生异构化。
外形
以含有蔗糖的冻干粉末形式提供
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Block copolymer microdomains: a novel medium for enzymatic reactions
Gupte, A., et al.
Biotechnology Progress, 7, 348-354 (2012)
Meihe Zhang et al.
Biosensors & bioelectronics, 32(1), 288-292 (2011-12-31)
A novel cholesterol biosensor was prepared based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence (ECL). Firstly, l-cysteine-reduced graphene oxide composites were modified on the surface of a glassy carbon electrode. Then, gold nanoparticles (AuNPs) were self-assembled on it. Subsequently, cholesterol oxidase
Laura Caldinelli et al.
The Journal of biological chemistry, 280(24), 22572-22581 (2005-04-09)
Cholesterol oxidase from Brevibacterium sterolicum is a monomeric flavoenzyme catalyzing the oxidation and isomerization of cholesterol to cholest-4-en-3-one. This protein is a class II cholesterol oxidases, with the FAD cofactor covalently linked to the enzyme through the His(69) residue. In
Tania Pomili et al.
Biosensors, 11(11) (2021-11-26)
In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical
Porntip H Lolekha et al.
Clinica chimica acta; international journal of clinical chemistry, 339(1-2), 135-145 (2003-12-23)
Cholesterol oxidase is used for the determination of serum cholesterol. It can be derived from Streptomyces, Pseudomonas fluorescens, Cellulomonas, and Brevibacterium. This study compared the performance characteristics of four enzymes in the endpoint cholesterol determination. Using the Mega analyzer, we
实验方案
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。
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